In the field of Applied Immunohistochemistry (IHC) various methods are used to determine the expression of biomarkers in tissue. Immunohistochemistry is widely used in pathology laboratory as an aid in the differential diagnosis and classification of cancer, and for certain other diseases, including infections. The factors that influence the immunohistochemical staining result start in the surgery operating room and end at the interpretation of the stain by the pathologist, which ultimately leads to treatment decision by the oncologist. For those who are eager to learn applied immunohistochemistry protocols or under go any live project works can refer respective services in this field.