Presently NTHRYS covers various protocols under its Research Assistance Services

rDNA Technology

1. DNA Extraction from Human Blood
2. DNA Extraction from Bacteria
3. DNA Extraction from Plant Leaf
4. DNA Extraction from Chicken Liver
5. Primer designing using Bioinformatics Tools
6. Optimization of PCR parameters
7. PCR
8. Agarose Electrophoresis using 1 - 10 Kbp ladder
9. Extraction & purification of amplified DNA from Agarose gels using spin columns
10. Cultivation of pUC 18 vector bearing bacterial strain
11. Plasmid [pUC 18] isolation
12. Restriction digestion digestion of pUC18 vector using EcoRI
13. 5` End DNA modification of restriction digested plasmid sample [Addition of Poly Ts]
14. TA Cloning [PCR Product and sample obtained above]
15. DNA ligation
16. Cultivation of DH5 alpha cells and Competent cell preparation using cultivated DH5 alpha cells
17. Bacterial Transformation [using competent cells and cloned vector obtained above]
18. Blue white screening [checking for the transformed colonies]

Biochemistry

19. Analysis
    1. Protein Analysis
    2. Carbohydrate Analysis
    1. Hexose Assay
    2. Pentose Assay
    3. Disacchharide Assay
    4. Polysacchharide Assay
    3. Lipid Analysis
    1. Lipid Extraction
    1. Modified Bligh and Dyers Method for Phospholipid Extraction
    2. Folch Extraction
    2. Thin Layer Chromatography
    4. Nucleic Acid Analysis
20. Assays
1. Enzyme Assays
1. Enzyme Kinetics [Basics]
2. Amylase Assay
3. Protease Assay
21. Protein Purifications
1. Protein Precipitations
2. Column Chromatography
22. Protein Analysis
1. Protein Estimations
1. Absorbance Assays
2. Colorimetric Assays
3. Commercial Protein Assays
2. Spectrometric Analysis
3. SDS PAGE
4. Gel Staining
1. Coomassie Blue Staining
2. Silver Staining
23. Lipid Extractions
1. Grey Method for Phosphatidylinositol Phosphate Extraction
2. Modified Alex Brown Method for Phosphatidylinositol Phosphate Extraction
3. Hexane Extraction for Neutral Lipids
4. Glycolipid Extraction
24. Immunprecipitations
25. Medical Plant Extraction studies
1. Medical Plant Extraction using Soxhlet Apparatus
2. Hydrextractions
3. Methanolic Extractions
4. Hexane Extractions
5. Ethanolic Extractions
26. Phytochemical Analysis
27. HPLC
28. GC
29. Western Blotting
30. Enzyme Assays
1. Lipid Kinase Assays
2. Protein Kinase Assays
3. Protein Tyrosine Phasphatase Assay
4. Alkaline Phosphatase Assay
5. Caspase Assay
31. Functional Assays
1. Apoptosis Assay
2. XTT Cell Proliferation Assay
3. Chemotaxis Assay
4. Matrigel Invasion or Cheoinvasion Assay
32. Mammalian Cell Culturing
1. Primary Cell Isolation & culturing
2. Splenocyte Isolation
3. Isolation of Peripheral Blood Lymphocytes
33. Tissue Culture
1. Adherent Cells
2. Suspension Cells
3. Cell Maintenance
4. Cell Counting
34. Anticarcinogenic Assays
1. MTT Assay
2. Tryphan Blue Assay

Microbiology

35. Isolation & Screening of soil microorganisms
36. Microbial stainings [Normal & Gram Staining]
37. Bacterial Motility test
38. Catalase Test
39. Mannitol Salt Agar Test
40. Blood Agar plates assay
41. Optochin sensistivity test
42. Bacitracin sensistivity test
43. CAMP Test
44. Bile esculin agar test
45. Nitrate broth test
46. Spirit blue agar test
47. Starch hydrolysis test
48. Coagulase test
49. Oxidase test
50. Sugar broth with durham's tubes test [Carbohydrate metabolism test]
    Glucose Test
    Sucrose Test
    Mannose Test
51. Methyl Red Voges Proskauer Test [MRVP Test]
52. Kliger's Iron Test
53. MacConkey Agar Test
54. Simmon's Citrate Test
55. Ureas Test
56. Sulfur Indole motility media test
57. Indole Test
58. Kliger's Iron Test

Animal Tissue Culture

59. Cultivation of Human Bone Marrow Stem Cells
60. Cultivation of Human Umbilical Cord Blood Stem Cells
61. Cultivation of Human Cardiomyocytes
62. Cultivation of HeLa Cells
63. Preparation and Use of Conditional Media [ Using Human Cardiomyocytes ]
64. Isolation and cultivation of aphid cells
65. Isolation and cultivation of fibroblasts from human tooth extracts

Plant Biotechnology

66. Aseptic culture techniques for establishment and maintenance of cultures
67. Preparation of stock solutions of MS basal medium and plant growth regulator stocks
68. Micropropagation of Tobacco plant by leaf disc culture
69. Micropropagation of Rice by indirect organogenesis from embryo
70. Preparation of competent cells of E. coli for harvesting plant transformation vector
71. Transformation of competent cells of E. coli with plant transformation vectors
72. Small scale plasmid preparation from E. coli
73. DNA check run by Agarose Electrophoresis
74. Restriction digestion of insert plasmid and binary vector
75. Electroelution of insert DNA from agarose gel slice
76. Mobilization of recombinant Ti plasmid from common laboratory host (E. coli) to an Agrobacterium tumefaciens strain
77. Agrobacterium tumefaciens-mediated plant transformation
78. Direct DNA delivery to plant by Particle Bombardment
79. Isolation of plant genomic DNA by modified CTAB method
80. Molecular analysis of putative transformed plants by Polymerase Chain Reaction

Immunotechnology

81. Extraction of IgG [Immunoglobulin G] from plasma / serum
82. Purification of extracted Immunoglobulins [Using Dialysis process]
83. Pepsin digestion and purification of digested IgG
84. Preparation of Antigens for Immunizations [including Adjuvant selection strategies]
85. SDS PAGE
86. ELISA [Sandwich ELISA - Qualitative]
87. RID
88. DID [Ouchterlony]
89. Immunization of Mice or Rabbit

Applied Immunology

Immunoassays

90. Competitive Radio Immunoassay [RIA]
91. Enzyme Linked Immunosorbent Assay [ELISA]
92. Solid-phase radioimmunoassay for cell-surface antigens

Isolation / Preparation / Production of Cells of Immune System


93. Isolation of normal peritoneal macrophages
94. Preparation of lymphocytes from blood
95. Preparation of lymphocytes from solid lymphoid organs
96. Production of human T-lymphocyte lines

Plant Tissue Culturing

97. Preparation of customized media and stock solution for selected plant tissue culturing experimentations
98. Preparation of Explants
99. Callus initiation and Maintenance in selected plants
100. Shoot & Root Induction in selected plants

Nanobiotechnology

101. Coating Antibodies [IgG] to Carbonanofibers
102. Coating of antibody coated carbon nanofibers to gold surface
103. Preparation of liposomal nanomedicines
104. Preparation of carbon nanofibers and liposomal conjugates

Aeromicrobiology

105. Air Sampling using Rotorod sampler
106. Air Sampling using Burkard sampler
107. Air Sampling using Anderson sampler
108. Enumeration of Fungi collected from Air Samples
109. Enumeration of Bacteria collected from air samples
110. Enumeration of total airborne bacteria, yeast and mold
111. Slide culture technique for fungi
112. Simple & Gram staining of Microorganisms
113. Cultivation & Isolation of single colonies of bacteria and fungi and storage
114. Isolation of Total DNA from isolated bacteria & Identification
115. Isolation of Total DNA from isolated fungi & Identification

Applied Immunochemistry

116. Human Tissue Collection & preparation
117. Tissue Fixation
118. Tissue Sectioning
119. Tissue Preservation
120. Sample / Tissue Labelling using IHC markers
121. Immunohistochemistry staining
122. Microscopic Observation, Photography & Data Analysis
123. Trouble Shooting

Clinical Immunology

124. C-Reactive Protein Test [To identify the presence of inflammation]
125. Erythrocyte Sedimentation Rate Test [To detect the presence of inflammation caused by one or more conditions]
126. Serum Autoantibody Assay [To check the presence of autoantibodies in blood]
127. Periodic acid–Schiff [PAS] staining [Staining macrophages in Erythroleukemia]
128. Total WBC Count
129. Differential WBC Count
130. Platelet Count
131. RBC Count
132. Systemic Lupus Erythematosus Diagnostic test
133. Rheumatoid Arthritis dignostic test

General Immunology

134. Antigen design
135. Human Thymus Cell Antigen preparation
136. Host Selection preparation for Immunization
137. Selection of Adjuvant for Antigen & Complete Antigen Preparation
138. Immunization Schedule
139. Testing Bleeds using ELISA
140. Bleeds & Plasma Collection
141. Antisera processing
142. Purification of Processed Antisera
143. Antisera Affinity testing against initial antigen used for Immunization

144. Spectro Metallurgical Testing

     1. CHEMICAL TESTING
        1. Optical Emission Spectrophotometer
           1. Ferrous Alloys Steels
              
              

              Low Alloy Steels, Stainless Steels, Free Cutting Steels, High carbon High chromium Steels, Tool Steels, Cast Iron and SG Iron

           2. Aluminium Alloys
              
              

              Al - Si Alloys
              Al - Mg - Zn Alloys
              

           3. Copper Alloys
              
              

              Chemical analysis of Brass, Leaded Bronze, Gunmetals, Phosphorous Bronze

     2. MECHANICAL TESTING by TUE-600C
     1. Tensile strength
        

        Yield strength
        % Elongation
        Bend and Rebend
        Testing of Tor Steels, Steels, Non-Ferrous metals
        

     2. Hardness

        Brinnel Hardness
        Rockwell Hardness
        

     3. Impact Charpy and Izod at room and sub zero temperatures
     3. SALTSPRAY TESTS by Neutral Salt Spray.

Select your desired field of Interest to view various services offered by NTHRYS in that field

Note: Use Ctrl + F in your browser to find your field of Interest from the below 200 fields.



1. aero microbiology


2. agri environmental


3. agricultural bioinformatics


4. agricultural biotechnology


5. agricultural microbiology


6. allergenomics


7. ancestry prediction


8. animal tissue culturing


9. applied genomics


10. applied immunochemistry


11. applied immunology


12. applied metabolomics


13. applied microbiology


14. applied proteomics


15. aquatic microbiology


16. bacteriology


17. bacteriophage genomics


18. biochemistry


19. bioengineering


20. bioinformatics


21. bioinorganic chemistry


22. biological engineering


23. biomedical


24. biomics


25. bioorganic chemistry


26. biophysics


27. bioprocess engineering


28. biorobotics


29. biosciences


30. biostatistics


31. biotechnology


32. blue biotechnology


33. cancer systems biology


34. cdisc sdtm


35. cdm


36. cellular microbiology


37. cheminformatics


38. clinical chemistry


39. clinical immunology


40. clinical medical bioinformatics


41. clinical trials


42. comparative genomics


43. computational biology


44. connectomics


45. cytomics


46. dairy technology


47. database development


48. drug designing


49. eco biotechnology


50. embedded systems


51. embryomics


52. enviromics


53. environmental bioinformatics


54. environmental biotechnology


55. enzymology


56. epigenomics


57. ethnicity prediction


58. evolutionary studies


59. exo microbiology


60. exomics


61. experimental biotechnology


62. exposomics


63. fermentation technology


64. food microbiology


65. foodomics


66. fuel biotechnology


67. functional domain prediction


68. functional genomics


69. fungal biotechnology


70. gene cloning genetical engineering


71. gene fusion


72. gene prediction


73. general biochemistry


74. general immunology


75. general microbiology


76. genome annotation


77. genomics


78. geo biotechnology


79. gis


80. glyco informaticss


81. glycomics


82. green biotechnology


83. hplc gc


84. immuno informatics


85. immuno technology


86. immunohistochemistry


87. immunopathology


88. impedance microbiology


89. industrial biochemistry


90. industrial microbiology


91. interactomics


92. interferomics


93. life sciences


94. lipidomics


95. marine biotechnology


96. mathematical modelling


97. matlab


98. mechatronics


99. medical biochemistry


100. medical biology


101. medical biotechnology


102. medical chemistry


103. medical microbiology


104. medical physics


105. metabolomics


106. metagenomics


107. microbiology


108. molecular agrobiology


109. molecular bioengineering


110. molecular biology


111. molecular biomarkers


112. molecular biophysics


113. molecular cancer research


114. molecular diagnostics


115. molecular dynamics


116. molecular ecology


117. molecular endocrinology


118. molecular eugenics


119. molecular farming


120. molecular gastronomy


121. molecular medicine


122. molecular microbiology


123. molecular modelling


124. molecular neurobiology


125. molecular oncology


126. molecular pathology


127. molecular pharmaceutics


128. molecular pharmacology


129. molecular phylogenetics


130. molecular pogramming


131. molecular veterinary diagnostics


132. molecular veterinary medicine


133. motif prediction


134. mycology


135. nano biotechnology


136. nano microbiology


137. nanotechnology


138. neuro electro dynamics


139. ngs


140. nuclear medicine


141. nutrigenomics


142. obesidomics


143. oral microbiology


144. organomics


145. pathology


146. pathway interactomics


147. perl programming


148. personalized medicine


149. petroleum microbiology


150. pharmaceutical biotechnology


151. pharmaceutical microbiology


152. pharmacogenetics


153. pharmacogenomics


154. pharmacology


155. pharmacovigilance


156. phenomics


157. phylodynamics


158. phylogenetics


159. phytochemomics


160. plant bioinformatics


161. plant biotechnology


162. plant breeding genetics


163. plant pathology


164. plant tissue culturing


165. plasma processing


166. population genetics


167. post harvest biology


168. protein folding


169. protein purification


170. proteomics


171. r programming


172. rdna technology


173. red biotechnology


174. regulomics


175. rna stucture prediction


176. robotics


177. sas


178. secretomics


179. sequence profiling


180. soil microbiology


181. stem cell transformations


182. structural bioinformatics


183. synthetic biological circuit


184. synthetic biology


185. synthetic intelligence


186. synthetic morphology


187. system biology


188. system microbiology


189. tissue engineering


190. toponomics


191. transcriptomics


192. translational genomics


193. translational research


194. translational research-informatics


195. vaccine biotechnology


196. vaccinology


197. veterinary biotechnology


198. veterinary microbiology


199. virology


200. water microbiology


201. weed biotechnology


202. white biotechnology
     


Modified on June 27th 2016






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