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    rDNA Technology

  1. DNA Extraction from Human Blood
  2. DNA Extraction from Bacteria
  3. DNA Extraction from Plant Leaf
  4. DNA Extraction from Chicken Liver
  5. Primer designing using Bioinformatics Tools
  6. Optimization of PCR parameters
  7. PCR
  8. Agarose Electrophoresis using 1 - 10 Kbp ladder
  9. Extraction & purification of amplified DNA from Agarose gels using spin columns
  10. Cultivation of pUC 18 vector bearing bacterial strain
  11. Plasmid [pUC 18] isolation
  12. Restriction digestion digestion of pUC18 vector using EcoRI
  13. 5` End DNA modification of restriction digested plasmid sample [Addition of Poly Ts]
  14. TA Cloning [PCR Product and sample obtained above]
  15. DNA ligation
  16. Cultivation of DH5 alpha cells and Competent cell preparation using cultivated DH5 alpha cells
  17. Bacterial Transformation [using competent cells and cloned vector obtained above]
  18. Blue white screening [checking for the transformed colonies]
  19. Biochemistry

  20. Analysis
    1. Protein Analysis
    2. Carbohydrate Analysis
      1. Hexose Assay
      2. Pentose Assay
      3. Disacchharide Assay
      4. Polysacchharide Assay
    3. Lipid Analysis
      1. Lipid Extraction
        1. Modified Bligh and Dyers Method for Phospholipid Extraction
        2. Folch Extraction
      2. Thin Layer Chromatography
    4. Nucleic Acid Analysis
  21. Assays
    1. Enzyme Assays
      1. Enzyme Kinetics [Basics]
      2. Amylase Assay
      3. Protease Assay
  22. Protein Purifications
    1. Protein Precipitations
    2. Column Chromatography
  23. Protein Analysis
    1. Protein Estimations
      1. Absorbance Assays
      2. Colorimetric Assays
      3. Commercial Protein Assays
    2. Spectrometric Analysis
    3. SDS PAGE
    4. Gel Staining
      1. Coomassie Blue Staining
      2. Silver Staining
  24. Lipid Extractions
    1. Grey Method for Phosphatidylinositol Phosphate Extraction
    2. Modified Alex Brown Method for Phosphatidylinositol Phosphate Extraction
    3. Hexane Extraction for Neutral Lipids
    4. Glycolipid Extraction
  25. Immunprecipitations
  26. Medical Plant Extraction studies
    1. Medical Plant Extraction using Soxhlet Apparatus
    2. Hydrextractions
    3. Methanolic Extractions
    4. Hexane Extractions
    5. Ethanolic Extractions
  27. Phytochemical Analysis
  28. HPLC
  29. GC
  30. Western Blotting
  31. Enzyme Assays
    1. Lipid Kinase Assays
    2. Protein Kinase Assays
    3. Protein Tyrosine Phasphatase Assay
    4. Alkaline Phosphatase Assay
    5. Caspase Assay
  32. Functional Assays
    1. Apoptosis Assay
    2. XTT Cell Proliferation Assay
    3. Chemotaxis Assay
    4. Matrigel Invasion or Cheoinvasion Assay
  33. Mammalian Cell Culturing
    1. Primary Cell Isolation & culturing
    2. Splenocyte Isolation
    3. Isolation of Peripheral Blood Lymphocytes
  34. Tissue Culture
    1. Adherent Cells
    2. Suspension Cells
    3. Cell Maintenance
    4. Cell Counting
  35. Anticarcinogenic Assays
    1. MTT Assay
    2. Tryphan Blue Assay

    Microbiology

  36. Isolation & Screening of soil microorganisms
  37. Microbial stainings [Normal & Gram Staining]
  38. Bacterial Motility test
  39. Catalase Test
  40. Mannitol Salt Agar Test
  41. Blood Agar plates assay
  42. Optochin sensistivity test
  43. Bacitracin sensistivity test
  44. CAMP Test
  45. Bile esculin agar test
  46. Nitrate broth test
  47. Spirit blue agar test
  48. Starch hydrolysis test
  49. Coagulase test
  50. Oxidase test
  51. Sugar broth with durham's tubes test [Carbohydrate metabolism test]
    Glucose Test
    Sucrose Test
    Mannose Test
  52. Methyl Red Voges Proskauer Test [MRVP Test]
  53. Kliger's Iron Test
  54. MacConkey Agar Test
  55. Simmon's Citrate Test
  56. Ureas Test
  57. Sulfur Indole motility media test
  58. Indole Test
  59. Kliger's Iron Test
  60. Animal Tissue Culture

  61. Cultivation of Human Bone Marrow Stem Cells
  62. Cultivation of Human Umbilical Cord Blood Stem Cells
  63. Cultivation of Human Cardiomyocytes
  64. Cultivation of HeLa Cells
  65. Preparation and Use of Conditional Media [ Using Human Cardiomyocytes ]
  66. Isolation and cultivation of aphid cells
  67. Isolation and cultivation of fibroblasts from human tooth extracts
  68. Plant Biotechnology

  69. Aseptic culture techniques for establishment and maintenance of cultures
  70. Preparation of stock solutions of MS basal medium and plant growth regulator stocks
  71. Micropropagation of Tobacco plant by leaf disc culture
  72. Micropropagation of Rice by indirect organogenesis from embryo
  73. Preparation of competent cells of E. coli for harvesting plant transformation vector
  74. Transformation of competent cells of E. coli with plant transformation vectors
  75. Small scale plasmid preparation from E. coli
  76. DNA check run by Agarose Electrophoresis
  77. Restriction digestion of insert plasmid and binary vector
  78. Electroelution of insert DNA from agarose gel slice
  79. Mobilization of recombinant Ti plasmid from common laboratory host (E. coli) to an Agrobacterium tumefaciens strain
  80. Agrobacterium tumefaciens-mediated plant transformation
  81. Direct DNA delivery to plant by Particle Bombardment
  82. Isolation of plant genomic DNA by modified CTAB method
  83. Molecular analysis of putative transformed plants by Polymerase Chain Reaction
  84. Immunotechnology

  85. Extraction of IgG [Immunoglobulin G] from plasma / serum
  86. Purification of extracted Immunoglobulins [Using Dialysis process]
  87. Pepsin digestion and purification of digested IgG
  88. Preparation of Antigens for Immunizations [including Adjuvant selection strategies]
  89. SDS PAGE
  90. ELISA [Sandwich ELISA - Qualitative]
  91. RID
  92. DID [Ouchterlony]
  93. Immunization of Mice or Rabbit
  94. Applied Immunology

    Immunoassays

  95. Competitive Radio Immunoassay [RIA]
  96. Enzyme Linked Immunosorbent Assay [ELISA]
  97. Solid-phase radioimmunoassay for cell-surface antigens
  98. Isolation / Preparation / Production of Cells of Immune System

  99. Isolation of normal peritoneal macrophages
  100. Preparation of lymphocytes from blood
  101. Preparation of lymphocytes from solid lymphoid organs
  102. Production of human T-lymphocyte lines
  103. Plant Tissue Culturing

  104. Preparation of customized media and stock solution for selected plant tissue culturing experimentations
  105. Preparation of Explants
  106. Callus initiation and Maintenance in selected plants
  107. Shoot & Root Induction in selected plants
  108. Nanobiotechnology

  109. Coating Antibodies [IgG] to Carbonanofibers
  110. Coating of antibody coated carbon nanofibers to gold surface
  111. Preparation of liposomal nanomedicines
  112. Preparation of carbon nanofibers and liposomal conjugates
  113. Aeromicrobiology

  114. Air Sampling using Rotorod sampler
  115. Air Sampling using Burkard sampler
  116. Air Sampling using Anderson sampler
  117. Enumeration of Fungi collected from Air Samples
  118. Enumeration of Bacteria collected from air samples
  119. Enumeration of total airborne bacteria, yeast and mold
  120. Slide culture technique for fungi
  121. Simple & Gram staining of Microorganisms
  122. Cultivation & Isolation of single colonies of bacteria and fungi and storage
  123. Isolation of Total DNA from isolated bacteria & Identification
  124. Isolation of Total DNA from isolated fungi & Identification
  125. Applied Immunochemistry

  126. Human Tissue Collection & preparation
  127. Tissue Fixation
  128. Tissue Sectioning
  129. Tissue Preservation
  130. Sample / Tissue Labelling using IHC markers
  131. Immunohistochemistry staining
  132. Microscopic Observation, Photography & Data Analysis
  133. Trouble Shooting
  134. Clinical Immunology

  135. C-Reactive Protein Test [To identify the presence of inflammation]
  136. Erythrocyte Sedimentation Rate Test [To detect the presence of inflammation caused by one or more conditions]
  137. Serum Autoantibody Assay [To check the presence of autoantibodies in blood]
  138. Periodic acid–Schiff [PAS] staining [Staining macrophages in Erythroleukemia]
  139. Total WBC Count
  140. Differential WBC Count
  141. Platelet Count
  142. RBC Count
  143. Systemic Lupus Erythematosus Diagnostic test
  144. Rheumatoid Arthritis dignostic test
  145. General Immunology

  146. Antigen design
  147. Human Thymus Cell Antigen preparation
  148. Host Selection preparation for Immunization
  149. Selection of Adjuvant for Antigen & Complete Antigen Preparation
  150. Immunization Schedule
  151. Testing Bleeds using ELISA
  152. Bleeds & Plasma Collection
  153. Antisera processing
  154. Purification of Processed Antisera
  155. Antisera Affinity testing against initial antigen used for Immunization
  156. Spectro Metallurgical Testing

    1. CHEMICAL TESTING
      1. Optical Emission Spectrophotometer
        1. Ferrous Alloys Steels

          Low Alloy Steels, Stainless Steels, Free Cutting Steels, High carbon High chromium Steels, Tool Steels, Cast Iron and SG Iron
        2. Aluminium Alloys

          Al - Si Alloys
          Al - Mg - Zn Alloys
        3. Copper Alloys

          Chemical analysis of Brass, Leaded Bronze, Gunmetals, Phosphorous Bronze
    2. MECHANICAL TESTING by TUE-600C
        1. Tensile strength
          Yield strength
          % Elongation
          Bend and Rebend
          Testing of Tor Steels, Steels, Non-Ferrous metals
        2. Hardness
          Brinnel Hardness
          Rockwell Hardness
        3. Impact Charpy and Izod at room and sub zero temperatures
    3. SALTSPRAY TESTS by Neutral Salt Spray.
  157. Select your desired field of Interest to view various services offered by NTHRYS in that field

    Note: Use Ctrl + F in your browser to find your field of Interest from the below 200 fields.

    1. aero microbiology

    2. agri environmental

    3. agricultural bioinformatics

    4. agricultural biotechnology

    5. agricultural microbiology

    6. allergenomics

    7. ancestry prediction

    8. animal tissue culturing

    9. applied genomics

    10. applied immunochemistry

    11. applied immunology

    12. applied metabolomics

    13. applied microbiology

    14. applied proteomics

    15. aquatic microbiology

    16. bacteriology

    17. bacteriophage genomics

    18. biochemistry

    19. bioengineering

    20. bioinformatics

    21. bioinorganic chemistry

    22. biological engineering

    23. biomedical

    24. biomics

    25. bioorganic chemistry

    26. biophysics

    27. bioprocess engineering

    28. biorobotics

    29. biosciences

    30. biostatistics

    31. biotechnology

    32. blue biotechnology

    33. cancer systems biology

    34. cdisc sdtm

    35. cdm

    36. cellular microbiology

    37. cheminformatics

    38. clinical chemistry

    39. clinical immunology

    40. clinical medical bioinformatics

    41. clinical trials

    42. comparative genomics

    43. computational biology

    44. connectomics

    45. cytomics

    46. dairy technology

    47. database development

    48. drug designing

    49. eco biotechnology

    50. embedded systems

    51. embryomics

    52. enviromics

    53. environmental bioinformatics

    54. environmental biotechnology

    55. enzymology

    56. epigenomics

    57. ethnicity prediction

    58. evolutionary studies

    59. exo microbiology

    60. exomics

    61. experimental biotechnology

    62. exposomics

    63. fermentation technology

    64. food microbiology

    65. foodomics

    66. fuel biotechnology

    67. functional domain prediction

    68. functional genomics

    69. fungal biotechnology

    70. gene cloning genetical engineering

    71. gene fusion

    72. gene prediction

    73. general biochemistry

    74. general immunology

    75. general microbiology

    76. genome annotation

    77. genomics

    78. geo biotechnology

    79. gis

    80. glyco informaticss

    81. glycomics

    82. green biotechnology

    83. hplc gc

    84. immuno informatics

    85. immuno technology

    86. immunohistochemistry

    87. immunopathology

    88. impedance microbiology

    89. industrial biochemistry

    90. industrial microbiology

    91. interactomics

    92. interferomics

    93. life sciences

    94. lipidomics

    95. marine biotechnology

    96. mathematical modelling

    97. matlab

    98. mechatronics

    99. medical biochemistry

    100. medical biology

    101. medical biotechnology

    102. medical chemistry

    103. medical microbiology

    104. medical physics

    105. metabolomics

    106. metagenomics

    107. microbiology

    108. molecular agrobiology

    109. molecular bioengineering

    110. molecular biology

    111. molecular biomarkers

    112. molecular biophysics

    113. molecular cancer research

    114. molecular diagnostics

    115. molecular dynamics

    116. molecular ecology

    117. molecular endocrinology

    118. molecular eugenics

    119. molecular farming

    120. molecular gastronomy

    121. molecular medicine

    122. molecular microbiology

    123. molecular modelling

    124. molecular neurobiology

    125. molecular oncology

    126. molecular pathology

    127. molecular pharmaceutics

    128. molecular pharmacology

    129. molecular phylogenetics

    130. molecular pogramming

    131. molecular veterinary diagnostics

    132. molecular veterinary medicine

    133. motif prediction

    134. mycology

    135. nano biotechnology

    136. nano microbiology

    137. nanotechnology

    138. neuro electro dynamics

    139. ngs

    140. nuclear medicine

    141. nutrigenomics

    142. obesidomics

    143. oral microbiology

    144. organomics

    145. pathology

    146. pathway interactomics

    147. perl programming

    148. personalized medicine

    149. petroleum microbiology

    150. pharmaceutical biotechnology

    151. pharmaceutical microbiology

    152. pharmacogenetics

    153. pharmacogenomics

    154. pharmacology

    155. pharmacovigilance

    156. phenomics

    157. phylodynamics

    158. phylogenetics

    159. phytochemomics

    160. plant bioinformatics

    161. plant biotechnology

    162. plant breeding genetics

    163. plant pathology

    164. plant tissue culturing

    165. plasma processing

    166. population genetics

    167. post harvest biology

    168. protein folding

    169. protein purification

    170. proteomics

    171. r programming

    172. rdna technology

    173. red biotechnology

    174. regulomics

    175. rna stucture prediction

    176. robotics

    177. sas

    178. secretomics

    179. sequence profiling

    180. soil microbiology

    181. stem cell transformations

    182. structural bioinformatics

    183. synthetic biological circuit

    184. synthetic biology

    185. synthetic intelligence

    186. synthetic morphology

    187. system biology

    188. system microbiology

    189. tissue engineering

    190. toponomics

    191. transcriptomics

    192. translational genomics

    193. translational research

    194. translational research-informatics

    195. vaccine biotechnology

    196. vaccinology

    197. veterinary biotechnology

    198. veterinary microbiology

    199. virology

    200. water microbiology

    201. weed biotechnology

    202. white biotechnology

    Modified on June 27th 2016




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NTHRYS NEWS

  1. Oct - 10th - 2015: Product design and standardization services are available under selected streams under Biotechnology at NTHRYS. Interested candidates please contact us with their detailed requirements.
  2. Oct - 1st - 2015: Tissue Engieering Projects are available from Dec 2015 at NTHRYS Hyderabad. Projects depended on 3D Printed ECM models are about to launch under this module
  3. Sep - 22nd - 2015: New methodologies under Biomedical Projects are underway to take their practical shape from January 2016 at NTHRYS. These projects invovle not only Medical Applications oriented one's but also Robobioics modules
  4. Sep - 15th - 2015: NTHRYS is launchig its Research Methodology Training Program from Dec 2015. Interested candidates please contact us.
  5. Sep - 10th - 2015: Thesis Assistance service / PhD Report Making Service / Dissertation report making enforcing Plagiarism rule using Turnitin Software is provided at NTHRYS Thesis Assistance Service
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  8. Aug - 17th - 2015: DST & DBT Project Proposal Assistance as well Project Maintenance and Management services are provided at NTHRYS. Please contact for more details
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  11. July - 22nd - 2015: NTHRYS has launched its pioneering project works under the domain Computational Neuroscience domain. Please call for more details
  12. July - 16th - 2015: Php based Artificial Intelligence algorithm designing assistance services are offered by NTHRYS. Please contact for more details
  13. July - 2nd - 2015: NTHRYS Launches Medical Projects Service under a broad range of Medical Sectors. Please visit the following Medical Projects Page for more details
  14. June - 30th - 2015: NTHRYS has reduced 20% on all Project & Training fee structures to Academic Candidates under the fields of Biotechnology & Bioinformatics
  15. June - 20th - 2015: NTHRYS Launched Compound Prediction Services for NMR, GC-MS, FTIR & UV Analysis Data. Contact Us for more details
  16. June - 2nd - 2015: NTHRYS now can assist Researchers & Academicians to procure Lab Animals from CPCSEA certified facilities located across the nation.
  17. May - 31st - 2015: NTHRYS now offers PhD Topic Selection, PhD Methodology design & PhD Practical Protocol Design assistance at affordable costs.
  18. May - 23rd - 2015: NTHRYS is proud to announce the launch of its much awaited Job Oriented Bioinformatics Training Program
  19. May - 15th - 2015: Commence of Training Program in Regression Analysis of Banking Data using SAS at NTHRYS
  20. May - 10th - 2015: Launch of Food Technology Projects from 2014 October
  21. April - 20th - 2015: Publication standard projects methodology assistance services offered by NTHRYS under the fields of Biotechnology, Bioinformatics, Embedded Systems, SAS, Environmental Sciences, Physiscs & Maths
  22. Feb - 8th - 2015: Launch of NGS Training Programme from 2015 January

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Tikamgarh,NTHRYS Protocols at Gandhinagar,NTHRYS Protocols at Bundi,NTHRYS Protocols at Ballia,NTHRYS Protocols at Darbhanga,NTHRYS Protocols at Gumla,NTHRYS Protocols at Jalandhar,NTHRYS Protocols at Central Delhi,NTHRYS Protocols at Shimla,NTHRYS Protocols at Kakinada,NTHRYS Protocols at Krishnagiri,NTHRYS Protocols at Chitradurga,NTHRYS Protocols at Palakkad,NTHRYS Protocols at Gadchiroli,NTHRYS Protocols at Gajapati,NTHRYS Protocols at Durg,NTHRYS Protocols at Chhatarpur,NTHRYS Protocols at Jamnagar,NTHRYS Protocols at Chittorgarh,NTHRYS Protocols at Balrampur,NTHRYS Protocols at East Champaran,NTHRYS Protocols at Hazaribag,NTHRYS Protocols at Kapurthala,NTHRYS Protocols at Sirmaur,NTHRYS Protocols at Kadapa,NTHRYS Protocols at Madurai,NTHRYS Protocols at Davangere,NTHRYS Protocols at Pathanamthitta,NTHRYS Protocols at Gondia,NTHRYS Protocols at Ganjam,NTHRYS Protocols at Gariaband,NTHRYS Protocols at Panna,NTHRYS Protocols at Junagadh,NTHRYS Protocols at Churu,NTHRYS Protocols at Banda,NTHRYS Protocols at Gaya,NTHRYS Protocols at Jamtara,NTHRYS Protocols at Ludhiana,NTHRYS Protocols at Solan,NTHRYS Protocols at Anantapur,NTHRYS Protocols at Nagapattinam,NTHRYS Protocols at Dharwad,NTHRYS Protocols at Thiruvananthapuram,NTHRYS Protocols at Hingoli,NTHRYS Protocols at Jagatsinghpur,NTHRYS Protocols at Jashpur,NTHRYS Protocols at Satna,NTHRYS Protocols at Kutch,NTHRYS Protocols at Dausa,NTHRYS Protocols at Barabanki,NTHRYS Protocols at Gopalganj,NTHRYS Protocols at Khuti,NTHRYS Protocols at ansa,NTHRYS Protocols at Una,NTHRYS Protocols at Nizamabad,NTHRYS Protocols at Namakkal,NTHRYS Protocols at Gadag,NTHRYS Protocols at Thrissur,NTHRYS Protocols at Jalgaon,NTHRYS Protocols at Jajapur (Jajpur),NTHRYS Protocols at Janjgir-Champa,NTHRYS Protocols at Rewa,NTHRYS Protocols at Kheda Dholpur,NTHRYS Protocols at Bareilly,NTHRYS Protocols at Jamui,NTHRYS Protocols at Koderma,NTHRYS Protocols at Moga,NTHRYS Protocols at Karimnagar,NTHRYS Protocols at Nilgiris,NTHRYS Protocols at Gulbarga,NTHRYS Protocols at Wayanad,NTHRYS Protocols at Jalna,NTHRYS Protocols at Jharsuguda,NTHRYS Protocols at Kondagaon,NTHRYS Protocols at Sidhi,NTHRYS Protocols at Mehsana,NTHRYS Protocols at Dungarpur,NTHRYS Protocols at Basti,NTHRYS Protocols at Jehanabad,NTHRYS Protocols at Latehar,NTHRYS Protocols at Mohali,NTHRYS Protocols at Khammam,NTHRYS Protocols at Perambalur,NTHRYS Protocols at Hassan,NTHRYS Protocols at Kolhapur,NTHRYS Protocols at Kalahandi,NTHRYS Protocols at Korba,NTHRYS Protocols at Singrauli,NTHRYS Protocols at Narmada,NTHRYS Protocols at Hanumangarh,NTHRYS Protocols at Bijnor,NTHRYS Protocols at Kaimur,NTHRYS Protocols at Lohardaga,NTHRYS Protocols at Muktsar,NTHRYS Protocols at Ramagundam,NTHRYS Protocols at Pudukkottai,NTHRYS Protocols at Haveri,NTHRYS Protocols at Latur,NTHRYS Protocols at Kandhamal,NTHRYS Protocols at Koriya,NTHRYS Protocols at Shahdol,NTHRYS Protocols at Navsari,NTHRYS Protocols at Jaipur,NTHRYS Protocols at Budaun,NTHRYS Protocols at Katihar,NTHRYS Protocols at Pakur,NTHRYS Protocols at Nawanshahr,NTHRYS Protocols at Eluru,NTHRYS Protocols at Ramanathapuram,NTHRYS Protocols at Kodagu,NTHRYS Protocols at Mumbai City,NTHRYS Protocols at Kendrapara,NTHRYS Protocols at Kanker,NTHRYS Protocols at Anuppur,NTHRYS Protocols at Panchmahal,NTHRYS Protocols at Jaisalmer,NTHRYS Protocols at Bulandshahr,NTHRYS Protocols at Khagaria,NTHRYS Protocols at Palamu,NTHRYS Protocols at Patiala,NTHRYS Protocols at Vizianagaram,NTHRYS Protocols at Salem,NTHRYS Protocols at Kolar,NTHRYS Protocols at Mumbai Sub-urban,NTHRYS Protocols at Kendujhar (Keonjhar),NTHRYS Protocols at Kabirdham,NTHRYS Protocols at Umaria,NTHRYS Protocols at Patan,NTHRYS Protocols at Jalor,NTHRYS Protocols at Chandauli,NTHRYS Protocols at Kishanganj,NTHRYS Protocols at Paschim singhbhum,NTHRYS Protocols at Rupnagar,NTHRYS Protocols at Proddatur,NTHRYS Protocols at Sivaganga,NTHRYS Protocols at Koppal,NTHRYS Protocols at Nagpur,NTHRYS Protocols at Khordha,NTHRYS Protocols at Mahasamund,NTHRYS Protocols at Katni,NTHRYS Protocols at Porbandar,NTHRYS Protocols at Jhalawar,NTHRYS Protocols at Chitrakoot,NTHRYS Protocols at Lakhisarai,NTHRYS Protocols at Purbi singhbhum,NTHRYS Protocols at Sangrur,NTHRYS Protocols at Nandyal,NTHRYS Protocols at Thanjavur,NTHRYS Protocols at Mandya,NTHRYS Protocols at Nanded,NTHRYS Protocols at Koraput,NTHRYS Protocols at Mungeli,NTHRYS Protocols at Damoh,NTHRYS Protocols at Rajkot,NTHRYS Protocols at Jhunjhunu,NTHRYS Protocols at Deoria,NTHRYS Protocols at Madhepura,NTHRYS Protocols at Ramgarh,NTHRYS Protocols at Tarn Taran,NTHRYS Protocols at Mahbubnagar,NTHRYS Protocols at Theni,NTHRYS Protocols at Mysore,NTHRYS Protocols at Nandurbar,NTHRYS Protocols at Malkangiri,NTHRYS Protocols at Narayanpur,NTHRYS Protocols at Dindori,NTHRYS Protocols at Sabarkantha,NTHRYS Protocols at Jodhpur,NTHRYS Protocols at Etah,NTHRYS Protocols at Madhubani,NTHRYS Protocols at Ranchi,NTHRYS Protocols at Ongole,NTHRYS Protocols at Thoothukudi,NTHRYS Protocols at North Kannada,NTHRYS Protocols at Nashik,NTHRYS Protocols at Mayurbhanj,NTHRYS Protocols at Raigarh,NTHRYS Protocols at Jabalpur,NTHRYS Protocols at Surat,NTHRYS Protocols at Karauli,NTHRYS Protocols at Etawah,NTHRYS Protocols at Munger,NTHRYS Protocols at Sahibganj,NTHRYS Protocols at Adoni,NTHRYS Protocols at Tiruchirappalli,NTHRYS Protocols at Shimoga,NTHRYS Protocols at Osmanabad,NTHRYS Protocols at Nabarangpur,NTHRYS Protocols at Rajnandgaon,NTHRYS Protocols at Sagar,NTHRYS Protocols at Surendranagar,NTHRYS Protocols at Kota,NTHRYS Protocols at Faizabad,NTHRYS Protocols at Muzaffarpur,NTHRYS Protocols at Sareikela and kharsawan,NTHRYS Protocols at Madanapalle,NTHRYS Protocols at Tirunelveli,NTHRYS Protocols at Dakshina Kannada,NTHRYS Protocols at Parbhani,NTHRYS Protocols at Nayagarh,NTHRYS Protocols at Raipur,NTHRYS Protocols at Vidisha,NTHRYS Protocols at Tapi,NTHRYS Protocols at Nagaur,NTHRYS Protocols at Farrukhabad,NTHRYS Protocols at Nalanda,NTHRYS Protocols at Simdega,NTHRYS Protocols at Chittoor,NTHRYS Protocols at Tiruvallur,NTHRYS Protocols at Tumkur,NTHRYS Protocols at Nuapada,NTHRYS Protocols at Surajpur,NTHRYS Protocols at Raisen,NTHRYS Protocols at Vadodara,NTHRYS Protocols at Nagaur,NTHRYS Protocols at Fatehpur,NTHRYS Protocols at Nawada,NTHRYS Protocols at Machilipatnam,NTHRYS Protocols at Tiruvannamalai,NTHRYS Protocols at Udupi,NTHRYS Protocols at Raigad,NTHRYS Protocols at Puri,NTHRYS Protocols at Sukma,NTHRYS Protocols at Bhopal,NTHRYS Protocols at Valsad,NTHRYS Protocols at Pali,NTHRYS Protocols at Firozabad,NTHRYS Protocols at Patna,NTHRYS Protocols at Tenali,NTHRYS Protocols at Tiruvarur,NTHRYS Protocols at Yadgir,NTHRYS Protocols at Ratnagiri,NTHRYS Protocols at Rayagada,NTHRYS Protocols at Surguja,NTHRYS Protocols at Rajgarh,NTHRYS Protocols at Pratapgarh,NTHRYS Protocols at Gautam,NTHRYS Protocols at Purnia,NTHRYS Protocols at Mancherial,NTHRYS Protocols at Vellore,NTHRYS Protocols at Chikkaballapur,NTHRYS Protocols at Sangli,NTHRYS Protocols at Sambalpur,NTHRYS Protocols at Shajapur,NTHRYS Protocols at Rajsamand,NTHRYS Protocols at Ghaziabad,NTHRYS Protocols at Rohtas,NTHRYS Protocols at Chirala,NTHRYS Protocols at Viluppuram,NTHRYS Protocols at Satara,NTHRYS Protocols at Subarnapur (Sonepur),NTHRYS Protocols at Mandsaur,NTHRYS Protocols at Sawai Madhopur,NTHRYS Protocols at Ghazipur,NTHRYS Protocols at Saharsa,NTHRYS Protocols at Nalgonda,NTHRYS Protocols at Virudhunagar,NTHRYS Protocols at Sindhudurg,NTHRYS Protocols at Sundargarh(Sundergarh),NTHRYS Protocols at Narsimhapur,NTHRYS Protocols at Sikar,NTHRYS Protocols at Gonda,NTHRYS Protocols at Samastipur,NTHRYS Protocols at Hindupur,NTHRYS Protocols at Tiruppur,NTHRYS Protocols at Solapur,NTHRYS Protocols at Chhindwara,NTHRYS Protocols at Sirohi,NTHRYS Protocols at Gorakhpur,NTHRYS Protocols at Saran,NTHRYS Protocols at Bhimavaram,NTHRYS Protocols at Thane,NTHRYS Protocols at Seoni,NTHRYS Protocols at Sri Ganganagar,NTHRYS Protocols at Hamirpur,NTHRYS Protocols at Sheikhpura,NTHRYS Protocols at Srikakulam,NTHRYS Protocols at Wardha,NTHRYS Protocols at Mandla,NTHRYS Protocols at Tonk,NTHRYS Protocols at Hardoi,NTHRYS Protocols at Sheohar,NTHRYS Protocols at Adilabad,NTHRYS Protocols at Washim,NTHRYS Protocols at Balaghat,NTHRYS Protocols at Udaipur,NTHRYS Protocols at Hathras,NTHRYS Protocols at Sitamarhi,NTHRYS Protocols at Guntakal,NTHRYS Protocols at Yavatmal,NTHRYS Protocols at Betul,NTHRYS Protocols at Rajasthan,NTHRYS Protocols at Jalaun,NTHRYS Protocols at Siwan,NTHRYS Protocols at Dharmavaram,NTHRYS Protocols at Hoshangabad,NTHRYS Protocols at Jaunpur,NTHRYS Protocols at Supaul,NTHRYS Protocols at Kothagudem,NTHRYS Protocols at Sehore,NTHRYS Protocols at Jhansi,NTHRYS Protocols at Vaishali,NTHRYS Protocols at Gudivada,NTHRYS Protocols at Harda,NTHRYS Protocols at Jyotiba,NTHRYS Protocols at West Champaran,NTHRYS Protocols at Narasaraopet,NTHRYS Protocols at Dewas,NTHRYS Protocols at Kannauj,NTHRYS Protocols at Siddipet,NTHRYS Protocols at Indore,NTHRYS Protocols at Kanpur Dehat,NTHRYS Protocols at Suryapet,NTHRYS Protocols at Ujjain,NTHRYS Protocols at Kanpur,NTHRYS Protocols at Miryalaguda,NTHRYS Protocols at Ratlam,NTHRYS Protocols at Kaushambi,NTHRYS Protocols at Jagtial,NTHRYS Protocols at Jhabua,NTHRYS Protocols at Kheri,NTHRYS Protocols at Tadepalligudem,NTHRYS Protocols at Dhar,NTHRYS Protocols at Kushinagar,NTHRYS Protocols at Chilakaluripet,NTHRYS Protocols at Khargone (West Nimari),NTHRYS Protocols at Lalitpur,NTHRYS Protocols at Khandwa (East Nimari),NTHRYS Protocols at Lucknow,NTHRYS Protocols at Bhurhanpur,NTHRYS Protocols at Maharajganj,NTHRYS Protocols at Barwani,NTHRYS Protocols at Mahoba,NTHRYS Protocols at Alirajpur,NTHRYS Protocols at Mainpuri,NTHRYS Protocols at Neemuch,NTHRYS Protocols at Mathura,NTHRYS Protocols at Mau,NTHRYS Protocols at Meerut,NTHRYS Protocols at Mirzapur,NTHRYS Protocols at Moradabad,NTHRYS Protocols at Muzaffarnagar,NTHRYS Protocols at Pilibhit,NTHRYS Protocols at Pratapgarh,NTHRYS Protocols at RaeBareli,NTHRYS Protocols at Rampur,NTHRYS Protocols at Saharanpur,NTHRYS Protocols at Sant kabir,NTHRYS Protocols at Sant Ravidas,NTHRYS Protocols at Shahjahanpur,NTHRYS Protocols at Shravasti,NTHRYS Protocols at Siddharthnagar,NTHRYS Protocols at Sonbhadra,NTHRYS Protocols at Sultanpur,NTHRYS Protocols at Unnao,NTHRYS Protocols at Varanasi
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