Aero-Microbiome & Airborne Microbial Surveillance — Hands-on

Design and run robust aero-microbiome studies for buildings, hospitals, cities and industrial plants. Learn air sampling hardware and protocols (filters, impactors, impingers, cyclones) , prevent contamination in ultra–low biomass contexts, execute 16S/ITS and shotgun workflows, and integrate meteorology & dispersion data for public-health or compliance reporting. Produce stakeholder-ready maps, alerts and SOP-driven, reproducible deliverables.

Aero-Microbiome & Airborne Microbial Surveillance

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Session Index

Session 1 — Air Sampling Design & Ultra–Low Biomass QC Session 2 — Amplicon/Shotgun Pipelines for Aerosols Session 3 — Source Tracking, Pathogens & Risk Indices Session 4 — Meteorology, Trajectories & Geo-Alert Dashboards

Session 1

Fee: Rs 8800

Air Sampling Design & Ultra–Low Biomass QC

Sampling media & devices

filters (PTFE, quartz) , cassettes impactors/impingers/cyclones PM_2.5/PM₁₀ size cuts

Study design & metadata

flow rates, volumes & durations indoor vs outdoor, HVAC zones MIxS/ENVO fields

Ultra–low biomass contamination control

field/kit blanks & negatives clean rooms & reagent QC DNA yield & inhibition checks

Session 2

Fee: Rs 11800

Amplicon/Shotgun Pipelines for Aerosols

Amplicon workflows

16S/ITS/18S marker choices DADA2/UNOISE; LULU curation IDTAXA/naive Bayes/BLAST LCA

Shotgun specifics & low-input tips

host/plant/fungal ribosomal depletion assembly/profiling (kraken2/MetaPhlAn) ARG/virulence panels (optional)

Decontamination & QA

decontam (prevalence/frequency) min-depth & replicate concordance balance/CLR-aware stats

Session 3

Fee: Rs 14800

Source Tracking, Pathogens & Risk Indices

Source apportionment

SourceTracker/Bayesian mixing indoor vs outdoor & HVAC urban/industrial/agri sources

Pathogen & allergen panels

clinically relevant fungi/bacteria qPCR/ddPCR assays thresholding & reporting

Risk & comfort indices

IAQ + microbiome composites occupancy/ventilation effects intervention what-ifs (HEPA/ACH)

Session 4

Fee: Rs 18800

Meteorology, Trajectories & Geo-Alert Dashboards

Meteorology integration

winds, RH, temperature AQI/PM overlays seasonality & events

Trajectories & dispersion (overview)

HYSPLIT back-trajectories Gaussian plume heuristics source hotspot mapping

Geo dashboards & reporting

QGIS/leaflet tiles alerts & thresholds SOPs & audit trails

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Aero-Microbiome & Airborne Microbial Surveillance Training | Sampling, Low-Biomass QC, Source Tracking & Geo-Alerts

Aero-Microbiome & Airborne Microbial Surveillance — Hands-on