Amplicon 16S 18S ITS Sequencing & QC — Hands-on

Develop end to end competence in marker gene (16S, 18S, ITS) amplicon workflows. You will design panels, plan barcoding and library prep, understand run layouts and apply rigorous QC to ensure reliable downstream taxonomic profiling and diversity analysis.

Amplicon 16S 18S ITS Sequencing and QC

Help Desk · WhatsApp

Session Index

Session 1 — Marker Gene Panels & Primer Design Session 2 — DNA Extraction, PCR & Barcoding Session 3 — Sequencing Layouts & Run Planning Session 4 — QC, Controls & Preprocessing

Session 1

Fee: Rs 8800

Marker Gene Panels & Primer Design

Marker gene basics for microbiome profiling

16S rRNA bacterial panels ITS for fungi 18S eukaryotic targets

Hypervariable regions and coverage trade offs

V1V2 / V3V4 / V4 amplicon length vs platform taxonomic resolution

Primer design and in silico evaluation

degenerate primers off target checks mock communities for validation

Session 2

Fee: Rs 11800

DNA Extraction, PCR & Barcoding

DNA extraction strategies for diverse matrices

stool / swab / soil / water mechanical and chemical lysis inhibitors and clean up

PCR optimization and amplification bias control

cycle number and annealing polymerase choice template input and inhibitors

Indexing, barcoding and plate layouts

single vs dual index index hopping awareness sample sheets and tracking

Session 3

Fee: Rs 14800

Sequencing Layouts & Run Planning

Platforms, read structures and throughputs

MiSeq and NextSeq class read length vs insert PhiX and run balance

Multiplexing and lane / flow cell planning

expected depth per sample pilot runs and optimization run level metadata

File formats and run outputs

BCL to FASTQ demultiplexing reports basic run QC summaries

Session 4

Fee: Rs 18800

QC, Controls & Preprocessing

Read level QC and trimming strategies

Theory + guided practical

Chimera checks, control evaluation and run sign off

negative / positive controls mock communities contamination flags

Deliverables: run QC pack and SOP bundle

QC reports and plots library prep and sequencing SOPs checklists for future runs

PDF

Amplicon 16S 18S ITS Sequencing & QC Training | Library Prep, Barcoding, Quality Control

Amplicon 16S 18S ITS Sequencing & QC — Hands-on