Antibody Modelling — Paratope/Epitope & Affinity Maturation — Hands-on

Build and optimize antibody models from sequence all the way to in-silico affinity maturation. This module covers antibody architecture, CDR numbering, paratope–epitope mapping, antibody–antigen docking and rational mutation strategies with basic developability and liability checks.

Antibody Modelling — Paratope/Epitope & Affinity Maturation

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Session Index

Session 1 — Antibody Architecture, CDRs & Paratope/Epitope Basics Session 2 — Antibody Modelling & CDR Loop Conformations Session 3 — Epitope Mapping, Docking & Affinity Estimation Session 4 — In-Silico Affinity Maturation & Developability

Session 1

Fee: Rs 22800

Antibody Architecture, CDRs & Paratope/Epitope Basics

Antibody structure and region definitions

VH/VL, CH/CL domains framework vs CDR regions Fab, Fc and formats overview

Numbering schemes and CDR definitions

Kabat / Chothia / IMGT schemes CDR boundaries and H3 special case tools for automated numbering

Paratope and epitope fundamentals

paratope residue composition linear vs conformational epitopes contact types and footprint size

Session 2

Fee: Rs 26800

Antibody Modelling & CDR Loop Conformations

Template based antibody modelling

germline and template selection framework grafting concepts light/heavy chain orientation

CDR loop modelling strategies

canonical classes for CDRs H3 loop conformational diversity loop sampling and refinement ideas

Geometry, clashes and quick validation checks

interface clashes and bumps backbone geometry and Ramachandran exposed liabilities inspection

Session 3

Fee: Rs 30800

Epitope Mapping, Docking & Affinity Estimation

Epitope mapping approaches

sequence and structure based predictors surface exposure and flexibility metrics use of experimental hints when available

Antibody–antigen docking concepts

paratope and epitope constraints global vs local docking ideas interface clustering and scoring

Affinity estimation and contact analysis

contact maps and interaction fingerprints in-silico alanine scanning ideas relative affinity ranking concepts

Session 4

Fee: Rs 33800

In-Silico Affinity Maturation & Developability

Designing mutations for affinity improvement

paratope residue hot-spot analysis focused CDR libraries maintaining epitope specificity

Basic in-silico developability checks

surface charge and hydrophobicity liabilities (deamidation, oxidation motifs) self-association risk indicators

Deliverables: antibody design and maturation report

ranked variant panel with rationale structure and interface figures experiment ready mutation suggestions

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Antibody Modelling Paratope/Epitope & Affinity Maturation Training | CDR Design, Binding & Optimization

Antibody Modelling — Paratope/Epitope & Affinity Maturation — Hands-on