Biophysical Properties — Stability, Solubility & Aggregation — Hands-on

Quantify and engineer biophysical behavior of proteins before going to the bench. This module connects structure, sequence and environment to stability, solubility and aggregation risk using in-silico biophysics, mutational scanning and developability-style profiling for enzymes, antibodies and other biologics.

Biophysical Properties — Stability, Solubility & Aggregation

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Session Index

Session 1 — Stability Concepts & In-Silico Probes Session 2 — Solubility, Aggregation & Developability Flags Session 3 — Mutational Scanning & Stability Engineering Session 4 — Biophysical Profiles, Reporting & Case Studies

Session 1

Fee: Rs 20800

Stability Concepts & In-Silico Probes

Thermodynamic vs kinetic stability for proteins

ΔG, T_m, unfolding curves two-state vs multi-state models environmental effects (pH, salt, cosolvents)

Sequence & structural determinants of stability

core packing and hydrophobic effect salt bridges and hydrogen bond networks loops, termini and disorder

In-silico stability probes and quick checks

empirical stability predictors packing scores and strain indicators simple MD / minimization sanity checks

Session 2

Fee: Rs 24800

Solubility, Aggregation & Developability Flags

Determinants of solubility and self-association

surface charge and pI hydrophobic patches colloidal vs conformational stability

Aggregation hotspots and prediction tools

linear aggregation motifs β-sheet prone regions exposed hydrophobics and interfaces

Developability-style in-silico flagging

charge–hydrophobicity plots liquid formulation risk indicators ranking variants by risk profile

Session 3

Fee: Rs 28800

Mutational Scanning & Stability Engineering

In-silico mutational scanning strategies

single point ΔΔG predictions saturation mutagenesis maps ranking stabilizing vs destabilizing changes

Design motifs for stability and solubility

core optimization and cavity filling disulfide and salt-bridge engineering surface charge and hydrophobic tuning

Balancing stability with activity and expression

avoidance of active-site perturbations expression host considerations shortlisting variants for experimental testing

Session 4

Fee: Rs 31800

Biophysical Profiles, Reporting & Case Studies

Putting stability, solubility and aggregation together

multi-metric biophysical dashboards traffic-light developability views comparing wild-type vs variants

Case studies: enzymes, antibodies and industrial proteins

stability improvement under process conditions reducing aggregation in biologics integrating experimental feedback

Deliverables: biophysical profile & design recommendation pack

summary tables and plots variant ranking and rationale slide-ready figures for stakeholders

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Biophysical Properties Stability, Solubility & Aggregation Training | Stability Engineering & Developability

Biophysical Properties — Stability, Solubility & Aggregation — Hands-on