Capstone — End-to-End Metabolomics/Fluxomics Project — Hands-on

Bring together everything you have learned across metabolomics, lipidomics and fluxomics into a single end to end project. In this capstone you will scope a biological question, work from RAW files through QC, statistics, pathway and flux interpretation, and finally deliver a fully documented, reproducible project package suitable for manuscripts or stakeholder review.

Capstone — End-to-End Metabolomics/Fluxomics Project

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Session Index

Session 1 — Project Scoping, Datasets & Experimental Context Session 2 — Processing, QC & Feature Matrices Session 3 — Statistics, Pathways & Flux Views Session 4 — Capstone Delivery: Report & Reproducible Package

Session 1

Fee: Rs 8800

Project Scoping, Datasets & Experimental Context

Selecting and framing the capstone question

disease, nutrition, toxicology or biotech scenario primary hypotheses and endpoints defining success criteria for the project

Understanding experimental design and metadata

groups, time points and interventions QC layout, batches and run order covariates and confounders to track

Assembling RAW files and prior preprocessing outputs

LC–MS/GC–MS/NMR RAWs and vendor formats peak lists, feature tables and logs project folder structure and naming

Session 2

Fee: Rs 11800

Processing, QC & Feature Matrices

Rebuilding or auditing the processing pipeline

peak picking, alignment and deconvolution check normalisation and transformation choices documenting all key parameters

QC metrics, batch assessment and correction

pooled QC performance and RSD filters run order drift and batch visualisation batch correction and post QC checks

Final analysis ready feature matrices

feature filtering and missingness handling log, scaling and transformation steps saving clean matrices with metadata keys

Session 3

Fee: Rs 14800

Statistics, Pathways & Flux Views

Core statistics and multivariate summaries

PCA and clustering for quality and trends group comparisons with FDR control effect size and volcano style summaries

Pathway, network and enrichment interpretation

mapping features to metabolites and pathways pathway enrichment and topology views overlaying results on metabolic networks

Fluxomics perspective (for tracer datasets)

basic EMU or model based summaries (overview) connecting isotopologue patterns to flux changes integrating steady state and flux level insights

Session 4

Fee: Rs 18800

Capstone Delivery: Report & Reproducible Package

Structuring the capstone story for stakeholders

background, methods, results and conclusions

Figures, tables and slide ready visualisations

QC, stats and pathway figure panels key tables for metabolites and fluxes summary slide deck outline

Reproducible project and handover package

notebook or script driven analysis flow data dictionary and environment file checklist for manuscript or client delivery

PDF

Capstone — End-to-End Metabolomics/Fluxomics Project Training | From RAW Files to Publication-Ready

Capstone — End-to-End Metabolomics/Fluxomics Project — Hands-on