Flux Balance Analysis & Constraint-Based Integration (FBA/FVA) — Hands-on

Link metabolite changes to network level behavior. This module introduces constraint-based models, Flux Balance Analysis (FBA) , Flux Variability Analysis (FVA) and simple strategies to integrate metabolomics or flux measurements so that you can formulate mechanistic hypotheses on pathway usage, bottlenecks and rerouting in cells and microbial systems.

Flux Balance Analysis & Constraint-Based Integration (FBA/FVA)

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Session Index

Session 1 — Metabolic Networks & Constraint-Based Model Basics Session 2 — Flux Balance Analysis (FBA) & Flux Variability (FVA) Session 3 — Integrating Metabolomics / Flux Data with FBA/FVA Session 4 — Mini Capstone: Toy Network FBA/FVA Study & Report

Session 1

Fee: Rs 8800

Metabolic Networks & Constraint-Based Model Basics

From pathway maps to mathematical network models

reactions, metabolites and compartments stoichiometric matrix idea exchange and transport reactions

Constraint-based modelling assumptions and scope

steady state flux balance concept flux bounds and reversibility what is captured vs omitted (kinetics)

Overview of genome scale models and toy networks

small core pathways vs whole cell models resources such as BiGG conceptually motivation for flux simulations

Session 2

Fee: Rs 11800

Flux Balance Analysis (FBA) & Flux Variability (FVA)

Setting up an FBA problem on a toy metabolic network

objective function idea (growth, ATP) linear programming viewpoint feasible region and optimal fluxes

Interpreting FBA solutions and sensitivity concepts

bottlenecks and active pathways shadow price intuition (high level) alternative optima and redundancy

Flux Variability Analysis (FVA) to explore solution space

minimum and maximum flux ranges rigid vs flexible reactions linking FVA results to robustness ideas

Session 3

Fee: Rs 14800

Integrating Metabolomics / Flux Data with FBA/FVA

Using uptake, secretion and growth data as FBA constraints

bounds from measured rates (concept) scenario setup for different media checking feasibility vs data

Overlaying metabolomics signatures on FBA solutions

linking metabolites to reactions increased pools vs expected flux shifts hypothesis generation from mismatches

Conceptual overview of expression and 13C data integration

expression guided constraints (high level) consistency with tracer-based fluxes caveats and limitations of integration

Session 4

Fee: Rs 18800

Mini Capstone: Toy Network FBA/FVA Study & Report

Setting up and analysing an FBA/FVA experiment on a toy model

Theory + Practical

Comparing different scenarios and interpreting flux re-routing

baseline vs perturbed constraints pathway usage and bottleneck plots link back to hypothetical metabolomics data

Deliverables: model file outline, flux tables & interpretation note

toy network and constraints description (PDF/HTML) FBA/FVA result table (CSV/TSV) short narrative on pathway level conclusions

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Flux Balance Analysis & Constraint-Based Integration (FBA/FVA) Training | Metabolic Networks & Fluxomics

Flux Balance Analysis & Constraint-Based Integration (FBA/FVA) — Hands-on