Gel-Based Proteomics — 2D Gel SDS-PAGE and Spots — Hands-on

Gain practical skills in gel-based proteomics using 2D gel electrophoresis (IEF + SDS-PAGE) for protein separation, visualization and spot-level analysis. You will learn end-to-end workflows from sample preparation, rehydration and IEF to second-dimension separation, staining, imaging, spot picking and in-gel digestion for downstream identification by MS.

Gel-Based Proteomics — 2D Gel SDS-PAGE and Spots

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Session Index

Session 1 — 2D Gel Proteomics Concepts & Design Session 2 — Sample Prep, Rehydration & IEF Session 3 — SDS-PAGE, Staining & Imaging Session 4 — Spot Picking & Mini Capstone

Session 1

Fee: Rs 8800

2D Gel Proteomics Concepts & Experimental Design

Overview of gel-based proteomics vs LC–MS workflows

1D vs 2D electrophoresis IEF + SDS-PAGE separation expression profiling & biomarkers

Experimental design for 2D gel studies

biological & technical replicates dye choice & staining strategy reference gels & normalization

Instrumentation & consumables overview

IEF system & IPG strips SDS-PAGE units imaging scanners & software

Session 2

Fee: Rs 11800

Sample Preparation, Rehydration & IEF

Protein extraction for 2D gels

chaotropes & detergents removing salts & nucleic acids compatible buffers for IEF

IPG strip rehydration & sample loading

linear vs nonlinear pH gradients passive / active rehydration sample volume & protein load

IEF run programming & QC

stepwise voltage ramp focusing criteria strip handling after IEF

Session 3

Fee: Rs 14800

Second-Dimension SDS-PAGE, Staining & Imaging

Equilibration and SDS-PAGE casting

DTT / iodoacetamide steps gradient gels vs single % strip placement & sealing

Gel staining and visualization options

Coomassie / silver staining fluorescent stains (concepts) background reduction & contrast

Imaging and basic gel quality assessment

scanner settings & dynamic range streaking & spot resolution archiving image files

Session 4

Fee: Rs 18800

Spot Picking, In-Gel Digestion & Mini Capstone

Spot detection concepts and picking strategy

Theory + Practical (demo / guided)

In-gel digestion workflow for MS identification

excising spots & destaining trypsin digestion in-gel peptide extraction & cleanup overview

Mini capstone: from 2D gel to MS-ready spot digest

experiment worksheet gel image & spot map handover sheet for MS core

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Gel-Based Proteomics Training | 2D Gel SDS-PAGE, Staining & Spot Analysis

Gel-Based Proteomics — 2D Gel SDS-PAGE and Spots — Hands-on