Glycan Biosynthesis Pathways and Glycoenzymes — Hands-on

Gain a pathway level view of how glycans are built and remodeled in cells. This module walks through ER and Golgi glycan biosynthesis, the organization of glycosyltransferases and glycosidases, donor substrates, and how genetic or process level changes in glycoenzymes alter glycoprotein and glycolipid profiles in health, disease and bioprocessing.

Glycan Biosynthesis Pathways and Glycoenzymes

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Session Index

Session 1 — Fundamentals of Glycan Biosynthesis Session 2 — N-glycan, O-glycan and Glycolipid Pathways Session 3 — Glycoenzymes, Specificity and Regulation Session 4 — Mini Capstone: Pathway Mapping and Perturbation

Session 1

Fee: Rs 8800

Fundamentals of Glycan Biosynthesis

Cellular landscape of glycan biosynthesis

ER and Golgi compartments lumen, membrane and cytosolic sides secretory pathway overview

Sugar nucleotide donors and transporters

UDP, GDP and CMP linked sugars nucleotide sugar synthesis routes Golgi transport and availability

General logic of stepwise glycan assembly

priming, elongation and capping branching and trimming cycles sequential enzyme action and order

Session 2

Fee: Rs 11800

N-glycan, O-glycan and Glycolipid Pathways

Core N glycan biosynthesis cascade

lipid linked oligosaccharide assembly transfer to Asn motifs in ER trimming and processing to complex forms

O glycan and glycosaminoglycan pathways

mucin type O GalNAc initiation core extension and branching heparan, chondroitin and dermatan sulfate chains

Glycolipid and ganglioside biosynthesis

ceramide based GSL backbones lacto, neo lacto and ganglio series roles in membranes and signaling

Session 3

Fee: Rs 14800

Glycoenzymes, Specificity and Regulation

Glycosyltransferase families and motifs

GT fold types and active site features donor and acceptor specificity Golgi localization and complex formation

Glycosidases and remodeling enzymes

ER quality control glycosidases lysosomal and extracellular enzymes turnover and salvage pathways

Regulation, genetic defects and engineering

congenital disorders of glycosylation cell line engineering for desired glycans pathway bottlenecks and flux points

Session 4

Fee: Rs 18800

Mini Capstone: Pathway Mapping and Perturbation

Constructing a glycan biosynthesis map for a protein of interest

Theory plus Practical

Simulating enzyme knockdown or overexpression scenarios

predicting impact on glycoform patterns linking pathway changes to phenotypes using simple spreadsheet or network tools

Deliverables: pathway diagram and perturbation summary

schematic of key glycoenzymes table of predicted glycan changes short interpretation for research or bioprocess

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Glycan Biosynthesis Pathways & Glycoenzymes Training | ER–Golgi Networks & Enzyme Logic

Glycan Biosynthesis Pathways and Glycoenzymes — Hands-on