Glycan Microarrays and Binding Assays — Hands-on

Develop practical understanding of glycan microarrays and related binding assays for decoding glycan–protein interactions. This module covers microarray platforms, glycan immobilization and printing, assay design with lectins, antibodies or pathogens, slide scanning, and data analysis workflows for generating interpretable glycan binding signatures.

Glycan Microarrays and Binding Assays

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Session Index

Session 1 — Fundamentals of Glycan Microarrays Session 2 — Printing, Immobilization & QC of Glycan Slides Session 3 — Binding Assays, Scanning & Data Analysis Session 4 — Mini Capstone: Glycan Microarray Binding Study

Session 1

Fee: Rs 8800

Fundamentals of Glycan Microarrays

Concept and applications of glycan microarrays

high throughput glycan–protein interaction mapping immune receptors, lectins and antibodies host–pathogen and cancer glycomics

Microarray platform chemistries and formats

glass slides and surface coatings covalent vs non covalent immobilization array printers and spotting technologies

Design of glycan libraries for arrays

natural, semi synthetic and synthetic glycans coverage of core motifs and linkages controls and reference spots

Session 2

Fee: Rs 11800

Printing, Immobilization & QC of Glycan Slides

Preparing glycan solutions for printing

buffer and concentration choices glycan derivatization and linkers stability and storage considerations

Array printing workflows and environmental control

contact and non contact printing spot morphology and uniformity blocking and post printing processing

Slide quality control and acceptance criteria

test binding with generic lectins signal to noise and background checks replicate spots and layout verification

Session 3

Fee: Rs 14800

Binding Assays, Scanning & Data Analysis

Assay design for glycan binding experiments

lectins, antibodies and serum samples pathogens, viruses and cells controls, replicates and titration series

Signal detection and slide scanning workflows

fluorescent labels and channels scanner settings and dynamic range image to spot intensity extraction

Basic data processing and binding signature generation

background subtraction and normalization log transforms and thresholding heatmaps and clustering of binding profiles

Session 4

Fee: Rs 18800

Mini Capstone: Glycan Microarray Binding Study

Planning a small glycan microarray experiment

Theory plus Practical

From raw scanner images to processed binding matrix

spot intensity table generation normalization across slides or batches identifying key glycan hits and patterns

Deliverables: array layout, binding signatures and report

CSV or XLSX binding matrix heatmaps and simple clustering plots short PDF or PPTX interpretation

PDF

Glycan Microarrays & Binding Assays Training | Platforms, Printing & Binding Signatures

Glycan Microarrays and Binding Assays — Hands-on