Glycan Release, Labeling and Enrichment Techniques — Hands-on

Learn the experimental steps required to isolate and prepare glycans from biotherapeutics and biological samples. This module covers N- and O-glycan release chemistries, fluorescent labeling strategies, cleanup and enrichment approaches that feed directly into LC–MS and glycomics pipelines.

Glycan Release, Labeling and Enrichment Techniques

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Session Index

Session 1 — Fundamentals of Glycan Release Session 2 — Fluorescent Labeling and Cleanup Session 3 — Glycan Enrichment Workflows Session 4 — Mini Capstone: End-to-End Sample Preparation

Session 1

Fee: Rs 8800

Fundamentals of Glycan Release

Sample types and pre treatment strategies

serum and plasma purified glycoproteins cell and tissue lysates

N-glycan release chemistries

enzymatic PNGase F and A denaturation and reduction buffer and pH considerations

O-glycan and other glycan release options

reductive beta elimination basics hydrazinolysis overview stability and side reactions

Session 2

Fee: Rs 11800

Fluorescent Labeling and Cleanup

Choosing labeling strategies and tags

2 AB and 2 AA labels procainamide and other tags fluorescence vs MS sensitivity

Reaction conditions and optimization

solvent and temperature reaction time and excess reagent avoiding degradation and loss

Post labeling cleanup workflows

SPE cartridges plate based cleanup recovery checks and QC

Session 3

Fee: Rs 14800

Glycan Enrichment Workflows

HILIC and solid phase enrichment

HILIC cartridges and tips graphitized carbon salt and solvent gradients

Lectin and affinity based enrichment of glycoforms

lectin columns and beads specificity and cross reactivity elution strategies

QC, recovery and troubleshooting steps

spike in controls chromatographic profiles common artifacts and fixes

Session 4

Fee: Rs 18800

Mini Capstone: End-to-End Sample Preparation

Designing a release, labeling and enrichment protocol

Theory plus Practical

Documenting steps, reagents and QC checkpoints

SOP style write up checklists and logs linking to LC MS run plans

Deliverables: protocol sheet and readiness for LC MS

PDF or DOC protocol sample tracking table pre injection QC summary

PDF

Glycan Release, Labeling & Enrichment Techniques Training | N- & O-Glycan Sample Preparation

Glycan Release, Labeling and Enrichment Techniques — Hands-on