Glycoengineering — Biotherapeutics & Cell-Surface Editing — Hands-on

Learn how to deliberately reprogram glycosylation on antibodies, cell therapies and other biologics. This module covers cell-line and enzyme based glycoengineering, chemoenzymatic remodeling, metabolic oligosaccharide engineering (MOE) and analytical readouts, enabling you to design, execute and interpret glycoengineering strategies for potency, safety, targeting and CQA control.

Glycoengineering — Biotherapeutics & Cell-Surface Editing

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Session Index

Session 1 — Principles of Glycoengineering for Biotherapeutics Session 2 — Cell-Line & Enzyme Based Glycoengineering Strategies Session 3 — Cell-Surface Editing, MOE & Functional Readouts Session 4 — Mini Capstone: Glycoengineering Design & CQA Plan

Session 1

Fee: Rs 8800

Principles of Glycoengineering for Biotherapeutics

Why glycoengineering? From natural variability to designed glycoforms

potency, half-life and safety reduced immunogenicity targeting and tissue distribution

Design space of glycoengineering interventions

terminal sialylation and fucosylation tuning branching, galactosylation and bisecting GlcNAc selection of target glyco metrics

Overview of glycoengineering toolkits

cell-line and pathway engineering chemoenzymatic remodeling metabolic oligosaccharide engineering (MOE)

Session 2

Fee: Rs 11800

Cell-Line & Enzyme Based Glycoengineering Strategies

Host cell engineering for desired glycoforms

CHO and HEK cell-line glycosylation backgrounds knockout/knock-in of glycosyltransferases feeding strategies and media supplements

Chemoenzymatic remodeling of glycans on purified biologics

endoglycosidase trimming and rebuilding sialyltransferases and fucosyltransferases batch-to-batch control and scalability

Analytics as feedback for process optimization

intact mass and subunit analysis released glycan and glycopeptide metrics linking process levers to glyco outputs

Session 3

Fee: Rs 14800

Cell-Surface Editing, MOE & Functional Readouts

Metabolic oligosaccharide engineering (MOE) on live cells

unnatural monosaccharide precursors bioorthogonal tags and click handles labeling and imaging of engineered glycans

Cell-surface editing for targeting and immune modulation

display of ligands and receptors via glycans tuning adhesion, trafficking and homing applications in CAR-T and cell therapies

Functional readouts and basic data analysis

lectin binding and flow cytometry cell-based potency and signaling assays linking glyco edits to phenotypic shifts

Session 4

Fee: Rs 18800

Mini Capstone: Glycoengineering Design & CQA Plan

Define a glycoengineering goal for a biologic or cell therapy

from desired function to glyco targets

Outline intervention strategy and analytics package

cell-line / enzyme / MOE options key LC-MS and cell-based readouts risk and feasibility considerations

Deliverables: design brief, CQA panel and reporting template

tabulated glyco CQAs and ranges figures for development reports short narrative for project teams

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Glycoengineering — Biotherapeutics & Cell-Surface Editing Training | Cell-Line, Enzyme & MOE Strategies

Glycoengineering — Biotherapeutics & Cell-Surface Editing — Hands-on