Glycopeptide Enrichment & Sample Prep — Hands-on

Develop practical, bench ready skills for glycopeptide focused sample preparation. This module walks through proteolysis design, enrichment chemistries (HILIC, lectin, TiO2/SAX) , cleanup and quality control so that downstream LC MS/MS glycoproteomics delivers confident site specific glycan information.

Glycopeptide Enrichment & Sample Prep

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Session Index

Session 1 — Samples, Proteolysis & Glycoprotein Strategy Session 2 — Glycopeptide Enrichment Chemistries Session 3 — Cleanup, Fractionation & QC Metrics Session 4 — Mini Capstone: Enrichment Workflow Design

Session 1

Fee: Rs 8800

Samples, Proteolysis & Glycoprotein Strategy

Sample types for glycopeptide analysis

purified antibodies and biologics serum / plasma / CSF cell and tissue lysates

Proteolysis design for glycopeptides

trypsin vs alternative proteases sequence coverage vs glyco site resolution enzyme to substrate ratios and incubation

Detergent removal and peptide ready extracts

SDS removal strategies FASP / S-Trap concepts preventing glycan loss in prep

Session 2

Fee: Rs 11800

Glycopeptide Enrichment Chemistries

HILIC based enrichment for glycopeptides

resin choices and binding conditions organic content and salt effects elution strategies and recovery

Lectin based enrichment strategies

lectin panels for motif targeting binding buffers and specificity competitive sugar elution concepts

Enrichment of sialylated and phosphopeptide like species

TiO2 / metal oxide overview SAX / SCX based approaches combining methods in workflows

Session 3

Fee: Rs 14800

Cleanup, Fractionation & QC Metrics

Desalting and detergent free glycopeptide pools

C18 tips and cartridges avoiding glycan loss in cleanup elution solvent choices

Fractionation options for complex samples

off line reversed phase fractions basic vs acidic fractionation balancing depth vs run time

QC metrics for enrichment success

% glycopeptide vs non glycopeptide recovery and reproducibility checks spike in and standard usage

Session 4

Fee: Rs 18800

Mini Capstone: Enrichment Workflow Design

Design a full glycopeptide enrichment workflow

from starting sample to LC MS ready fraction

Match enrichment strategy to biological question

site occupancy vs motif discovery sialylation vs core fucosylation focus limited material vs high throughput

Deliverables and documentation

workflow schematic step wise SOP style protocol QC checklist and acceptance criteria

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Glycopeptide Enrichment & Sample Prep Training | Proteolysis, Enrichment & QC for Glycoproteomics

Glycopeptide Enrichment & Sample Prep — Hands-on