Glycosaminoglycans — Hyaluronan Heparan & Chondroitin — Hands-on

Gain a practical understanding of glycosaminoglycans (GAGs) including hyaluronan, heparan and chondroitin chains, their sulfation patterns and analytical behavior. This module walks through sample prep, enzymatic digestion, LC-MS workflows and data analysis to generate quantitative GAG profiles that can be linked to biology, biomaterials or bioprocess changes.

Glycosaminoglycans — Hyaluronan Heparan & Chondroitin

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Session Index

Session 1 — GAG Classes, Structure & Chemistry Session 2 — Workflows for GAG Digestion & LC-MS Session 3 — Disaccharide Analysis & Sulfation Patterns Session 4 — Mini Capstone: GAG Profiles & Interpretation

Session 1

Fee: Rs 8800

GAG Classes, Structure & Chemistry

Overview of major GAG families and biosynthetic themes

hyaluronan heparan and heparin chondroitin and dermatan

Repeating units, linkage patterns and chain organization

uronic acid and hexosamine pairs linkage to core proteins domains and microheterogeneity

Chemical properties impacting analytics

charge density and sulfation conformation and flexibility stability and depolymerization

Session 2

Fee: Rs 11800

Workflows for GAG Digestion & LC-MS

Sample preparation for GAG extraction and cleanup

cell, tissue and biofluid sources proteoglycan enrichment basics desalting and removal of interferents

Enzymatic depolymerization to disaccharides

lyases for heparan and chondroitin hyaluronidases for hyaluronan reaction controls and stopping

LC-MS methods for GAG disaccharide profiling

HILIC and reverse phase options negative mode ESI MS and MS/MS internal standards and calibration

Session 3

Fee: Rs 14800

Disaccharide Analysis & Sulfation Patterns

Peak picking, alignment and identification of GAG disaccharides

m/z and retention time windows library and standard based ID handling isomeric disaccharides

Quantification and composition calculation per GAG class

area integration and response factors relative vs absolute amounts chain length and content estimates

Describing sulfation and pattern metrics

N and O sulfation distributions average charge per disaccharide indexes for domain patterning

Session 4

Fee: Rs 18800

Mini Capstone: GAG Profiles & Interpretation

Build a small GAG profiling workflow on example datasets

from LC-MS tables to composition summaries

Summarize GAG composition and sulfation across conditions

plots for class level changes heatmaps for disaccharide patterns linking shifts to biology or process

Deliverables and documentation set

curated disaccharide and GAG tables figures for reports or slides short narrative on key GAG changes

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Glycosaminoglycans — Hyaluronan Heparan & Chondroitin Training | Disaccharide Analysis & GAG Profiling

Glycosaminoglycans — Hyaluronan Heparan & Chondroitin — Hands-on