Glycosylation Types — N-linked, O-linked and GPI — Hands-on

Build a clear conceptual and practical understanding of the main protein glycosylation types that shape protein structure and function. This module covers N-linked, O-linked and GPI anchor glycosylation, including sequence motifs, biosynthetic pathways, structural consequences, and analytical readouts relevant to biotherapeutics, diagnostics and glycobiology research.

Glycosylation Types — N-linked, O-linked and GPI

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Session Index

Session 1 — Overview of Protein Glycosylation Types Session 2 — N-linked Glycosylation Motifs and Pathways Session 3 — O-linked Glycosylation and GPI Anchors Session 4 — Mini Capstone: Glycosylation Mapping Case Study

Session 1

Fee: Rs 8800

Overview of Protein Glycosylation Types

Glycosylation as a post translational modification

roles in folding and stability quality control and trafficking signaling and recognition

Classification of major glycosylation types

N linked vs O linked GPI anchors other less common linkages

Cellular compartments and enzymes involved

ER and Golgi localization glycosyltransferases and glycosidases overview of glycan processing steps

Session 2

Fee: Rs 11800

N-linked Glycosylation Motifs and Pathways

N linked consensus motifs and sequence context

N X S and N X T motifs motif variants and exceptions motif prediction in protein sequences

Co translational addition and ER processing of N glycans

lipid linked oligosaccharide assembly oligosaccharyltransferase action calnexin and calreticulin cycle

Golgi trimming and extension to complex N glycans

high mannose, hybrid and complex types branching and bisecting GlcNAc terminal sialylation and fucosylation

Session 3

Fee: Rs 14800

O-linked Glycosylation and GPI Anchors

Overview of O linked glycosylation types

mucin type O GalNAc O GlcNAc and nuclear cytoplasmic roles other O linked modifications

Enzymes and pathways for O linked glycosylation

GalNAc transferases and substrate selection extension to core structures regulation and crosstalk with phosphorylation

GPI anchor biosynthesis and protein attachment

GPI precursor assembly signal sequence recognition and attachment GPI anchored protein functions at the membrane

Session 4

Fee: Rs 18800

Mini Capstone: Glycosylation Mapping Case Study

Interpreting experimental data to classify glycosylation type

Theory plus Practical

Using sequence motifs and domain context for site prediction

motif scanning and filters integration with MS or NMR evidence curating a glycosylation site list

Deliverables: annotated protein map and glycosylation summary

protein schematic with N, O and GPI sites table of sites and evidence levels short interpretive report

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Glycosylation Types N-linked, O-linked & GPI Training | Motifs, Pathways & Functional Roles

Glycosylation Types — N-linked, O-linked and GPI — Hands-on