HTS Data Analysis, Hit Triage & Deconvolution — Hands-on

Turn high-throughput screening (HTS) data into a confident hit list and deconvoluted leads. This hands-on module covers plate QC (Z′, S/B, CV) , normalization and control design, hit picking under FDR, dose–response curve fitting (IC₅₀/EC₅₀/Hill) , interference/aggregator detection, SAR clustering, and orthogonal/confirmatory triage with transparent reporting.

HTS Data Analysis, Hit Triage & Deconvolution

Help Desk · WhatsApp

Session Index

Session 1 — Assay Design, Controls & Plate QC Session 2 — Normalization, Hit Calling & FDR Session 3 — Curve Fitting, Interference & SAR Session 4 — Mini Capstone: Deconvolution Pack

Session 1

Fee: Rs 19800

Assay Design, Controls & Plate QC

Assay & screening modes

biochemical / cell-based single-point / dose–response primary → confirm → orthogonal

Controls & plate QC

pos/neg/neutral controls Z′-factor, S/B, S/N, CV edge & drift diagnostics

Data integrity

barcode & map validation replicate logic audit & provenance

Session 2

Fee: Rs 22800

Normalization, Hit Calling & FDR Control

Normalization strategies

B-score/Loess/robust Z %control & fold-change batch/plate effects

Hit thresholds & statistics

z-score/median-abs dev p-values & BH/FDR replicate concordance

QC dashboards

heatmaps & scatter control trendlines outlier triage

Session 3

Fee: Rs 25800

Curve Fitting, Interference Filters & SAR Clustering

Dose–response modelling

4PL/variable slope IC₅₀/EC₅₀ & Hill curve class & flags

Interference, artifacts & aggregators

PAINS/Brenk alerts redox/fluorescence/colloids detergent shift checks

SAR & clustering

ECFP similarity & clusters scaffold series & medoids chemotype enrichment

Session 4

Fee: Rs 29800

Mini Capstone: Deconvolution & Decision Pack

End-to-end: QC → normalize → call hits → fit curves → filter → cluster

Theory + Practical

Orthogonal & confirmation plan

retest/cherry-pick sets mechanism controls solubility & DMSO checks

Deliverables

ranked hit list (CSV) QC & curve report (HTML/PDF) triage slides (PDF)

PDF

HTS Data Analysis, Hit Triage & Deconvolution | QC, Normalization, Curve Fitting & SAR

HTS Data Analysis, Hit Triage & Deconvolution — Hands-on