Immunoglycomics and Antibody Glycosylation — Hands-on

Understand how glycans modulate immune recognition and antibody effector function. This module focuses on IgG Fc glycosylation, immune receptor glycosylation, experimental workflows for profiling antibody glycans, and interpretation of glyco patterns in infection, autoimmunity and therapeutic antibody development.

Immunoglycomics and Antibody Glycosylation

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Session Index

Session 1 — Immune System and Glycosylation Basics Session 2 — Antibody Fc Glycosylation Patterns and Functions Session 3 — Experimental Workflows for Antibody Glycosylation Session 4 — Mini Capstone: IgG Glycosylation Profile and Interpretation

Session 1

Fee: Rs 8800

Immune System and Glycosylation Basics

Overview of innate and adaptive immunity

B and T cell roles antibodies and Fc receptors complement and innate sensors

Glycans in immune recognition and regulation

self versus non self discrimination pathogen associated glycan patterns immune checkpoint and receptor glycosylation

Basics of antibody structure and glycosylation sites

Fab and Fc regions IgG subclasses and N297 site other immunoglobulin classes

Session 2

Fee: Rs 11800

Antibody Fc Glycosylation Patterns and Functions

Common IgG Fc glycoforms and notation

G0, G1, G2 and bisecting GlcNAc core fucosylation and afucosylation sialylation and galactosylation levels

Impact of Fc glycans on effector functions

ADCC, CDC and ADCP activity Fc gamma receptor binding profiles anti inflammatory versus pro inflammatory signatures

Antibody glycosylation in infection and autoimmunity

disease associated IgG glyco shifts vaccination and infection case examples therapeutic antibody design considerations

Session 3

Fee: Rs 14800

Experimental Workflows for Antibody Glycosylation

Sample preparation strategies for IgG glyco analysis

serum, plasma and purified IgG Protein A or G enrichment subclass or Fc fragment workflows

Released glycan, glycopeptide and intact mass approaches

PNGase F release and labeling HILIC and LC–MS glycan profiling site specific glycopeptide LC–MS/MS

Quantitation, normalization and QC metrics for IgG glycans

relative abundance of Fc glycoforms intra and inter batch comparisons basic system suitability checks

Session 4

Fee: Rs 18800

Mini Capstone: IgG Glycosylation Profile and Interpretation

From LC–MS data to IgG glycan profile table

Theory plus Practical

Summarizing Fc glycan features into functional scores

galactosylation, sialylation and fucosylation indexes heatmaps and radar plots of Fc signatures comparison across cohorts or time points

Deliverables: IgG glyco profile and functional interpretation summary

CSV or XLSX glycan abundance table plots for key Fc features short PDF or PPTX report for stakeholders

PDF

Immunoglycomics & Antibody Glycosylation Training | IgG Fc Glycans & Effector Functions

Immunoglycomics and Antibody Glycosylation — Hands-on