Immunoglycomics — Antibody Glycoforms & Fc Functions — Hands-on

Understand how antibody Fc glycosylation patterns shape immune effector functions, safety and potency. This module links IgG glycobiology and effector pathways with LC-MS based released glycan and glycopeptide workflows, and shows how to derive quantitative glycoform metrics that support immunology research, vaccine programs and biotherapeutic CQA panels.

Immunoglycomics — Antibody Glycoforms & Fc Functions

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Session Index

Session 1 — IgG Fc Glycobiology & Effector Pathways Session 2 — Workflows for Antibody Glycoform Characterization Session 3 — Glycoform Metrics, CQAs & Immuno Readouts Session 4 — Mini Capstone: Antibody Glycoform Panel & Report

Session 1

Fee: Rs 8800

IgG Fc Glycobiology & Effector Pathways

Architecture of IgG and the conserved Fc glycan site

Fc versus Fab glycosylation N linked glycan at Asn297 themes G0, G1, G2 and bisecting motifs

How Fc glycoforms modulate effector mechanisms

ADCC, CDC and ADCP concepts afucosylation and enhanced cytotoxicity galactosylation and complement activation

Immunological and clinical contexts for IgG glycans

autoimmune and inflammatory signatures vaccine response and infection profiles therapeutic antibodies and biosimilars

Session 2

Fee: Rs 11800

Workflows for Antibody Glycoform Characterization

Released Fc glycan profiling from serum or purified IgG

IgG purification and subclass enrichment basics enzymatic release and cleanup LC MS methods tuned to Fc glycans

Glycopeptide level analysis of Fc and Fab regions

protease choices and sequence coverage fragmentation for site resolved glycoforms multiplexed analysis of subclasses or clones

Top down and middle down perspectives on IgG glycans

intact mass and subunit level views linking intact patterns to detailed IDs fast screens versus deep characterization

Session 3

Fee: Rs 14800

Glycoform Metrics, CQAs & Immuno Readouts

Deriving Fc glycoform distributions from LC MS tables

G0F, G1F, G2F and related patterns afucosylation and galactosylation levels sialylation and bisecting GlcNAc fractions

Connecting glyco metrics to CQAs and bioassays

specification windows and target ranges bridging to ADCC and CDC outputs comparability between lots or cell lines

Statistics and visualization for immunoglycomics data

group comparisons for glycoform features correlation with titers or effector readouts heatmaps and profile plots for review

Session 4

Fee: Rs 18800

Mini Capstone: Antibody Glycoform Panel & Report

Build a small IgG glycoform panel on example datasets

from raw quant tables to Fc glyco metrics

Summarize differences across cohorts or production runs

comparability style plots and tables linking glyco shifts to effector hypotheses simple rules of thumb for interpretation

Deliverables and documentation set

curated glycoform distribution table review ready figures for teams short narrative on key Fc glyco insights

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Immunoglycomics — Antibody Glycoforms & Fc Functions Training | IgG Fc Glycans, ADCC & CQAs

Immunoglycomics — Antibody Glycoforms & Fc Functions — Hands-on