Immunoproteomics and Antibody Profiling — Hands-on

Build a conceptual understanding of immunoproteomics and antibody profiling. This module focuses on antigen–antibody interactions, serological assay thinking, immunocapture LC–MS ideas, and how antibody and antigen profiles can be interpreted as immune response signatures and biomarker candidates.

Immunoproteomics and Antibody Profiling

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Session Index

Session 1 — Immunology & Antibody Basics for Proteomics Session 2 — Serological Assays & Immunocapture Concepts Session 3 — Antibody Profiling, Specificity & Repertoire Ideas Session 4 — Mini Capstone: Immunoproteomics Study Design

Session 1

Fee: Rs 8800

Immunology & Antibody Basics for Proteomics

Core immunology concepts relevant to proteomics studies

innate vs adaptive ideas B cells, T cells and antibodies antigen presentation (high level)

Antibody structure, classes and effector function concepts

Fab and Fc regions idea IgG / IgM / IgA overview isotype and subclass thinking

Antigen–antibody binding and epitope concepts (overview)

affinity and avidity ideas linear vs conformational epitopes specificity vs cross reactivity awareness

Session 2

Fee: Rs 11800

Serological Assays & Immunocapture Concepts

Serological assay families at conceptual level

ELISA ideas lateral flow concept titer and cut off thinking

Immunocapture and immunoenrichment for LC–MS (overview only)

antibody as capture reagent idea pulling down antigens from complex matrices concept of improved selectivity & sensitivity

Controls, specificity checks and matrix effects (concepts)

blank and negative controls spike recovery idea cross reactivity awareness

Session 3

Fee: Rs 14800

Antibody Profiling, Specificity & Repertoire Ideas

Concepts of antibody profiling and immune signatures

antigen array and panel ideas patterns across patient groups autoantibody signature concepts

Specificity, cross reactivity and background binding (high level)

epitope overlap ideas off target binding awareness blocking and competition concepts

Overview of repertoire level and clonality thinking (conceptual)

diversity and clonal expansion ideas linking repertoire to antigen exposure (high level) integration with omics and clinical data (concepts)

Session 4

Fee: Rs 18800

Mini Capstone: Immunoproteomics Study Design

Conceptual design of a simple immunoproteomics project

Theory + Practical (planning oriented)

From clinical or biological question to assay and panel layout (idea level)

cohort and sample type thinking choice of antigens / antibodies at concept level readout and basic analysis plan

Mini capstone deliverables (design and narrative focused)

study design sheet outline simple antibody/antigen panel table short narrative linking profiles to hypotheses

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Immunoproteomics & Antibody Profiling Training | Serological & Immunocapture Concepts

Immunoproteomics and Antibody Profiling — Hands-on