In Silico Mutagenesis, Stability & Function Impact — Hands-on

Learn how to plan, run and interpret in silico mutagenesis so that stability and function impact of sequence changes are quantified and summarized clearly. This module focuses on mutation scanning concepts, structural context, stability like views, and functional reasoning so that you can propose, prioritize and defend mutation panels for experiments or design projects.

In Silico Mutagenesis, Stability & Function Impact

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Session Index

Session 1 — Mutagenesis & Stability Concepts Session 2 — In Silico Scanning & Stability Style Views Session 3 — Functional Impact & Mechanistic Reasoning Session 4 — Mini Capstone: Mutation Panel & Impact Pack

Session 1

Fee: Rs 8800

Mutagenesis & Stability Concepts

Motivations for in silico mutagenesis and scanning designs

rational engineering ideas risk assessment in clinical variants screening for stabilizing or activating changes

Stability style metrics and interpretation basics

delta like free energy notions relative vs absolute scales what counts as mildly vs strongly destabilizing

Structural context for mutations on proteins and complexes

core vs surface positions idea interface, active site and allosteric zones conservation and environment cues

Session 2

Fee: Rs 11800

In Silico Scanning & Stability Style Views

Designing mutation scans across domains and sites

alanine scanning ideas focused vs broad mutation sets balancing coverage with cost and time

Generating structure based stability style predictions conceptually

single point mutation evaluations interpreting positive vs negative scores relating predictions to local packing and contacts

Visualizing scans on sequences and structures for insight

heatmaps along sequence positions colouring structures by predicted impact spotting clusters of sensitive residues

Session 3

Fee: Rs 14800

Functional Impact & Mechanistic Reasoning

Linking stability changes to possible functional outcomes

loss of function vs gain of function ideas aggregation and misfolding risks buffer regions vs fragile structural cores

Active site, interface and allosteric zone mutations

catalytic and binding residue roles disrupting vs tuning interactions coupling to long range communication paths

Summarizing in silico evidence for experimental teams

shortlists of priority mutations tables with predicted impact and rationale flags for assay design and readouts

Session 4

Fee: Rs 18800

Mini Capstone: Mutation Panel & Impact Pack

Pick a protein or complex and define a mutagenesis objective

Theory + Practical

Design, run and summarize an in silico mutation panel

heatmaps and structural views of impact highlight stabilizing and risky options connect predictions to mechanism hypotheses

Deliverables: mutation panel tables, figures and interpretation note

ranked mutation list with comments sequence and structure annotated views recommendations for experiments or further modeling

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In Silico Mutagenesis, Stability & Function Impact Training | Scanning, Stability Views & Impact Stories

In Silico Mutagenesis, Stability & Function Impact — Hands-on