Integrative Structural Biology — CryoX EM NMR SAXS — Hands-on

Learn how to integrate Cryo-EM, X-ray crystallography, NMR and SAXS data into coherent structural biology workflows. From interpreting maps and restraints to building, refining and validating hybrid models, you will design integrative structural studies that leverage multiple experimental modalities for reliable, publication-ready insights into macromolecular assemblies.

Integrative Structural Biology — CryoX EM NMR SAXS

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Session Index

Session 1 — Modalities, Data Types & Resolutions Session 2 — Model Building & Refinement per Modality Session 3 — Hybrid / Integrative Modeling Workflows Session 4 — Validation, Deposition & Reporting

Session 1

Fee: Rs 12320

Modalities, Data Types & Resolutions

Cryo-EM, X-ray, NMR & SAXS in one view

what each technique measures resolution regimes and limits when to combine vs use alone

Core data formats and metadata concepts

maps, structure factors and restraints ideas NOE, RDC and other NMR observables (concepts) SAXS curves, q range and Guinier views

Experimental design for integrative studies

choosing complementary techniques sample considerations for each method planning for downstream modeling

Session 2

Fee: Rs 16520

Model Building & Refinement per Modality

X-ray crystallography model workflows (concepts)

electron density interpretation ideas refinement, R factors and B factors ligand and solvent modeling basics

Cryo-EM map fitting and refinement views

rigid body and flexible fitting ideas local resolution and filtering awareness map vs model consistency checks

NMR & SAXS guided modeling

using restraints and ensembles (concepts) fitting SAXS curves with models flexible and multi domain systems views

Session 3

Fee: Rs 20720

Hybrid / Integrative Modeling Workflows

Combining multiple restraints and data sources

maps, distances, crosslinks and SAXS together weighting and scoring ideas sampling conformational space concepts

Assemblies, complexes and flexible regions

multi chain and multi domain assemblies dealing with missing density or mobility coarse grained vs atomistic representations

Workflow patterns for integrative modeling

from input data to ranked models ensembles, clustering and representative states handoffs to MD and docking projects

Session 4

Fee: Rs 26320

Validation, Deposition & Reporting

Validation metrics for integrative models

geometry checks and stereochemistry fit to data and cross validation concepts model confidence and uncertainty summaries

Deposition standards and archives (concepts)

PDB, EMDB and related resources integrative / hybrid model deposition ideas metadata and annotation expectations

Figures, tables and stories for manuscripts & collaborators

multi modality figure panels cartoons, density overlays and SAXS fits clear model provenance and workflow diagrams

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Integrative Structural Biology — CryoX EM NMR SAXS Training | Hybrid Modeling & Multi-Modal Data

Integrative Structural Biology — CryoX EM NMR SAXS — Hands-on