LC-MS & GC-MS Workflows for Untargeted Metabolomics — Hands-on

Learn how to plan and execute robust LC–MS and GC–MS runs for untargeted metabolomics. This module connects chromatographic choices, ionization and acquisition settings, batch layout and QC strategy so that you can consistently generate high quality raw data for downstream peak detection, alignment and statistical analysis.

LC-MS & GC-MS Workflows for Untargeted Metabolomics

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Session Index

Session 1 — LC–MS / GC–MS Platforms & Untargeted Strategy Session 2 — Chromatography, Gradients & MS Acquisition Session 3 — Run Setup, Batches & Basic Troubleshooting Session 4 — Mini Capstone: Untargeted Run Plan & Methods Sheet

Session 1

Fee: Rs 8800

LC–MS / GC–MS Platforms & Untargeted Strategy

Instrument landscape for metabolomics LC–MS and GC–MS

triple quadrupole QTOF / Orbitrap (overview) quadrupole GC–MS

Untargeted vs targeted thinking in LC–MS / GC–MS studies

discovery profiling coverage vs sensitivity trade off panel and follow up design

Ionization and polarity modes for metabolite classes

ESI positive / negative APCI (concepts) polarity switching and coverage

Session 2

Fee: Rs 11800

Chromatography, Gradients & MS Acquisition

LC method basics for untargeted metabolomics

HILIC vs RP columns mobile phase additives gradient shape and length

GC method basics for metabolomics after derivatization

column selection temperature programs carrier gas and flow

MS acquisition modes for untargeted data

full scan high resolution data dependent MS and MS (overview) basic parameter ranges

Session 3

Fee: Rs 14800

Run Setup, Batches & Basic Troubleshooting

Preparing the sequence: samples, QCs, blanks and standards

conditioning injections interleaved pooled QCs system suitability and calibration

Monitoring key metrics during the run

retention time stability peak shape and intensity QC CVs and drift plots

Common LC–MS / GC–MS issues and first line fixes

clogging and pressure alerts signal loss and noise carryover and contamination

Session 4

Fee: Rs 18800

Mini Capstone: Untargeted Run Plan & Methods Sheet

Designing an LC–MS or GC–MS untargeted run for a cohort

Theory + Practical

Documenting chromatography, MS parameters and batch layout

method sheet for LC / GC sequence file plan QC and blank placements

Deliverables: method description, sequence map & QC summary

LC–MS / GC–MS methods sheet run-order and batch sheet (CSV) QC strategy documentation

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LC-MS & GC-MS Workflows for Untargeted Metabolomics Training | Chromatography & Acquisition

LC-MS & GC-MS Workflows for Untargeted Metabolomics — Hands-on