LC–MS and MS/MS for Glycan Profiling — Hands-on

Learn how to design and execute liquid chromatography–mass spectrometry workflows tailored for glycan analysis. This module walks through LC method selection, gradient design, MS and MS/MS acquisition strategies and basic QC checks for confident glycan profiling in research and biopharma labs.

LC–MS and MS/MS for Glycan Profiling

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Session Index

Session 1 — LC–MS Fundamentals for Glycans Session 2 — Chromatographic Methods and Gradients Session 3 — MS/MS Fragmentation and Data Acquisition Session 4 — Mini Capstone: Glycan LC–MS Profiling Workflow

Session 1

Fee: Rs 8800

LC–MS Fundamentals for Glycans

Instrument components and ionization basics

LC modules and pumps ESI source overview quadrupole and TOF concepts

Ions, adducts and charge states for glycans

protonated and sodiated species label dependent behavior in source decay awareness

Key LC–MS parameters and system suitability

flow rate and injection volume source temperature and voltages test mixes and performance checks

Session 2

Fee: Rs 11800

Chromatographic Methods and Gradients

Column chemistries for glycan separations

HILIC columns RP and mixed mode options particle size and dimensions

Mobile phases, buffers and additives

organic solvent choices pH and buffer systems MS friendly additives

Gradient design, run time and carry over control

shallow vs steep gradients wash and re equilibration steps blank and carry over checks

Session 3

Fee: Rs 14800

MS/MS Fragmentation and Data Acquisition

MS1 acquisition and scan strategies

full scan settings resolution and scan speed dynamic range considerations

Fragmentation methods for glycans

CID and HCD basics B/Y and cross ring ions label dependent patterns

DDA and targeted acquisition for glycans

top N methods and exclusion lists targeted MS/MS of known species basic DIA concepts for glycomics

Session 4

Fee: Rs 18800

Mini Capstone: Glycan LC–MS Profiling Workflow

Designing an LC–MS method for a glycan panel

Theory plus Practical

Running standards and real samples with QC

system suitability criteria retention time tracking signal quality checks

Deliverables: method sheet, run log and raw data overview

PDF or DOC method description run sequence table basic chromatogram snapshots

PDF

LC–MS & MS/MS for Glycan Profiling Training | HILIC Methods, Fragmentation & QC Workflows

LC–MS and MS/MS for Glycan Profiling — Hands-on