LC MS MS Acquisition Parameters & Best Practices — Hands-on

Learn to configure LC MS MS platforms for robust metabolomics and lipidomics data acquisition. From source tuning and chromatographic gradients to DDA/DIA and MRM/PRM methods, you will design runs that maximize coverage and data quality for downstream processing.

LC MS MS Acquisition Parameters & Best Practices

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Session Index

Session 1 — LC MS MS Platforms & Ionization Session 2 — Chromatographic Methods & Gradients Session 3 — MS MS Acquisition Modes (DDA/DIA/MRM/PRM) Session 4 — System Suitability, QC & Best Practices

Session 1

Fee: Rs 8800

LC MS MS Platforms & Ionization

LC MS MS hardware overview

triple quadrupole Q TOF and Orbitrap hybrid instruments

Ion sources and polarity switching

ESI and APCI basics positive and negative mode polarity switching trade offs

Source tuning and inlet conditions

spray voltage and gas flows temperature and desolvation contamination prevention

Session 2

Fee: Rs 11800

Chromatographic Methods & Gradients

Columns and stationary phases for metabolomics

reversed phase HILIC mixed mode options

Mobile phases and additives

buffer selection pH and volatility ionization friendly choices

Gradient design and flow parameters

gradient shape and length flow rate and backpressure column temperature control

Session 3

Fee: Rs 14800

MS MS Acquisition Modes (DDA/DIA/MRM/PRM)

Full scan and DDA setup

scan ranges top N and dynamic exclusion isolation windows

DIA windows and library friendly settings

fixed versus variable windows cycle time management collision energy schemes

MRM and PRM method design

transition selection dwell time and scheduling LOD and LOQ thinking

Session 4

Fee: Rs 18800

System Suitability, QC & Best Practices

System suitability tests for metabolomics

mixture and standard checks

Performance metrics and run monitoring

mass accuracy and resolution sensitivity and precision RT and peak shape checks

Maintenance, troubleshooting and documentation

common failure modes cleaning and upkeep method templates and logs

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LC MS MS Acquisition Parameters & Best Practices Training | DDA/DIA & MRM/PRM for Metabolomics

LC MS MS Acquisition Parameters & Best Practices — Hands-on