LC MS MS Methods — DDA DIA PRM SRM — Hands-on

Develop an intuitive understanding of the major LC–MS/MS acquisition strategies used in proteomics. This module focuses on how DDA, DIA, PRM and SRM work at a conceptual level, how method parameters influence coverage, sensitivity and throughput, and how to choose and plan acquisition modes for discovery and targeted studies.

LC MS MS Methods — DDA DIA PRM SRM

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Session Index

Session 1 — LC MS MS Fundamentals & Scan Logic Session 2 — DDA Methods & Optimization Concepts Session 3 — DIA & PRM Design Logic Session 4 — SRM Panels & Method Planning

Session 1

Fee: Rs 8800

LC MS MS Fundamentals & Scan Logic

Signal path and key terms in LC–MS/MS

MS1 and MS2 precursor and fragment ions scan events and cycles

Chromatography and mass spectrometry together

retention time and peak shape gradient overview resolution vs run time

Cycle time, duty cycle and trade offs

points across a peak coverage vs sensitivity throughput considerations

Session 2

Fee: Rs 11800

DDA Methods & Optimization Concepts

DDA precursor selection logic

intensity based top N view isolation window concept charge state preferences

Dynamic exclusion and sampling the chromatogram

repeat sequencing avoidance exclusion windows complex vs simple samples

Using pilot runs to refine DDA methods

adjusting gradients mass range choices cycle settings sanity checks

Session 3

Fee: Rs 14800

DIA & PRM Design Logic

DIA acquisition concepts

segmented mass window view coverage vs interference library based vs library free ideas

PRM for targeted quant conceptually

monitoring selected precursors high resolution MS2 focus panel size and cycle time

When to choose DDA vs DIA vs PRM

discovery vs verification cohort size and depth needs instrument and time constraints

Session 4

Fee: Rs 18800

SRM Panels & Method Planning

SRM concept for highly targeted assays

theory plus planning exercise

Transitions, scheduling and dwell time logic

precursor and fragment choice time window scheduling panel size vs data quality

Designing a simple acquisition plan across modes

linking study goals to modes instrument time budgeting documenting method settings

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LC MS MS Methods — DDA DIA PRM SRM Training | Acquisition Strategies for Proteomics

LC MS MS Methods — DDA DIA PRM SRM — Hands-on