Lectin Arrays, Microarrays & Binding Profiles — Hands-on

Learn how to design, run and interpret lectin array and glycan microarray assays for glycan profiling. This module covers probe and lectin panel selection, blocking and hybridization strategies, scanner setup, data extraction, normalization and downstream analysis to derive robust binding signatures for biologics, sera, cells and microbial samples.

Lectin Arrays, Microarrays & Binding Profiles

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Session Index

Session 1 — Lectins, Glycan Recognition & Panel Design Session 2 — Lectin Array & Microarray Wet Lab Workflows Session 3 — Data Extraction, Normalization & QC Session 4 — Mini Capstone: Binding Signatures & Reporting

Session 1

Fee: Rs 8800

Lectins, Glycan Recognition & Panel Design

Lectin biology and glycan binding specificities overview

binding to core, branch and terminal motifs overlapping and cross reactivity carbohydrate recognition domains basics

Designing lectin and glycan microarray panels

covering major glycan motif families controls and replicates on the array panels for biologics vs serum vs cells

Experimental design for binding profile studies

group sizes and replicates batching and plate layout positive and negative controls

Session 2

Fee: Rs 11800

Lectin Array & Microarray Wet Lab Workflows

Sample preparation and labeling strategies

protein and glycoprotein labeling concepts fluorescent vs alternative readouts avoiding signal saturation and loss

Blocking, hybridization and washing conditions

blocking buffers and additives incubation times and temperatures stringency and background reduction

Scanning, image acquisition and basic QC

scanner settings and dynamic range spot morphology inspection acceptance criteria per slide

Session 3

Fee: Rs 14800

Data Extraction, Normalization & QC

Spot finding and intensity extraction from array images

grid alignment and spot detection foreground vs background signals exporting raw intensity tables

Normalization within and across slides or batches

global scaling and reference spots per slide and per lectin adjustments handling array to array variability

QC metrics and diagnostic plots for arrays

signal distributions and boxplots correlation heatmaps and clustering flagging poor quality samples

Session 4

Fee: Rs 18800

Mini Capstone: Binding Signatures & Reporting

Build a simple lectin binding signature pipeline

from intensity tables to normalized profiles

Derive patterns and group differences from arrays

principal component and clustering views lectin level statistics linking patterns to glycan motifs

Deliverables and reporting templates

curated binding matrix table figures for presentations or manuscripts short narrative summary of key signatures

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Lectin Arrays, Microarrays & Binding Profiles Training | Experimental Design & Binding Analytics

Lectin Arrays, Microarrays & Binding Profiles — Hands-on