Lectinomics and Glycan-Receptor Interactions — Hands-on

Learn how lectins recognize specific glycan motifs and how these interactions can be systematically profiled and quantified. This module covers lectin families, lectin panel design, lectin blot/ELISA and cell based assays, along with data analysis strategies for mapping glycan–receptor interactions in immunity, host–pathogen biology and cancer research.

Lectinomics and Glycan-Receptor Interactions

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Session Index

Session 1 — Lectin Families and Binding Specificity Session 2 — Experimental Assays for Lectin–Glycan Interactions Session 3 — Data Analysis, Motif Calling & Network Views Session 4 — Mini Capstone: Lectinomics Interaction Map

Session 1

Fee: Rs 8800

Lectin Families and Binding Specificity

Overview of lectins and glycan recognition

definition and basic properties monovalent vs multivalent binding affinity vs avidity concepts

Major lectin families and exemplar ligands

C type, galectins, siglecs plant lectins (ConA, PNA, WGA) host and microbial lectins

Glycan motifs and structural determinants

terminal residues and linkages branching, fucosylation, sialylation influence of presentation and density

Session 2

Fee: Rs 11800

Experimental Assays for Lectin–Glycan Interactions

Classical lectin based detection methods

lectin blotting and staining lectin ELISA and plate assays flow cytometry with fluorescent lectins

Designing and using lectin panels

selecting lectins for key motifs controls and specificity checks competition and inhibition experiments

Quantitative binding readouts and Kd estimation basics

signal vs concentration curves simple binding models limitations of semi quantitative assays

Session 3

Fee: Rs 14800

Data Analysis, Motif Calling & Network Views

Processing lectin signal matrices

background correction and normalization replicate handling and QC thresholding and z scores

Motif level interpretation of lectin patterns

mapping lectins to glycan features co binding and motif co occurrence linking to underlying glycan classes

Visualizing glycan–lectin interaction networks

heatmaps and clustering of samples bipartite network views basic enrichment and pathway overlays

Session 4

Fee: Rs 18800

Mini Capstone: Lectinomics Interaction Map

Designing a small lectin panel experiment

Theory plus Practical

From raw signals to glycan interaction signatures

assembling a sample × lectin matrix normalization and motif calling identifying distinct glycan phenotypes

Deliverables: interaction map and interpretive summary

CSV or XLSX lectin response table heatmaps / network plots short PDF or PPTX report

PDF

Lectinomics & Glycan-Receptor Interactions Training | Assays, Motifs & Interaction Maps

Lectinomics and Glycan-Receptor Interactions — Hands-on