Lipidomics Workflows — Class-Level, Species-Level, Isomer Resolution — Hands-on

Build practical lipidomics workflows that go beyond simple class totals. This module focuses on LC–MS based lipidomics from class-level profiling through species-level detail and isomer-aware strategies, so you can design realistic experiments, choose appropriate internal standards, and interpret lipid changes with the right level of resolution for your study.

Lipidomics Workflows — Class-Level, Species-Level, Isomer Resolution

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Session Index

Session 1 — Lipidomics Foundations & Experimental Planning Session 2 — Class-Level & Semi-Quantitative Lipidomics Session 3 — Species-Level Detail & Isomer Resolution Concepts Session 4 — Mini Capstone: Lipidomics Workflow Design & Reporting

Session 1

Fee: Rs 8800

Lipidomics Foundations & Experimental Planning

Lipid classes, subclasses and basic nomenclature (concepts)

glycerophospholipids, sphingolipids, neutral lipids acyl/alkyl chains, headgroups class vs species level thinking

LC–MS based lipidomics platforms overview

reversed-phase vs HILIC ideas polarity switching and acquisition modes targeted vs untargeted overview

Designing lipidomics experiments and QC layout

sample randomization and run order pooled QC and reference mixtures coverage vs depth trade offs

Session 2

Fee: Rs 11800

Class-Level & Semi-Quantitative Lipidomics

Concepts of class-level lipid profiling

headgroup driven separation or detection summed class abundance pros and cons of class level views

Internal standards and semi-quantitation at class level

class-matched internal standards response factors (concepts) relative vs absolute reporting

Summarising and visualising class-level lipid changes

stacked bar and heatmap ideas ratio style metrics (e.g. SFA/PUFA) linking to biology and pathways

Session 3

Fee: Rs 14800

Species-Level Detail & Isomer Resolution Concepts

Moving from class sums to molecular species detail

chain length and unsaturation patterns species naming schemes (concept) biological interpretation at species level

Isomer issues: position, double bond and regiochemistry

sn-1/sn-2 position concepts double bond location and geometry ideas limitations of routine workflows

Chromatographic and MS/MS strategies for better resolution (overview)

separation of key subclasses diagnostic fragment concepts deciding the level of detail to target

Session 4

Fee: Rs 18800

Mini Capstone: Lipidomics Workflow Design & Reporting

Designing a realistic lipidomics workflow for a study question

Theory + Practical

Choosing appropriate resolution: class, species and isomer aspects

matching resolution to hypothesis balancing complexity and feasibility documenting limitations explicitly

Deliverables: workflow diagram, resolution plan & reporting template

lipidomics workflow outline (PDF/HTML) class/species/isomer resolution notes results and figure checklist for studies

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Lipidomics Workflows — Class-Level, Species-Level, Isomer Resolution Training | LC–MS Lipidomics Design

Lipidomics Workflows — Class-Level, Species-Level, Isomer Resolution — Hands-on