MALDI–TOF and LC–ESI for Oligosaccharide Analysis — Hands-on

Build hands-on skills in MALDI–TOF and LC–ESI based analysis of oligosaccharides from glycoproteins and complex samples. This module covers matrix and solvent choices, spotting and infusion techniques, acquisition and calibration routines, and interpretation of isotope patterns and adducts for confident structural assignments.

MALDI–TOF and LC–ESI for Oligosaccharide Analysis

Help Desk · WhatsApp

Session Index

Session 1 — MALDI–TOF Fundamentals for Oligosaccharides Session 2 — LC–ESI Workflows for Oligosaccharide Analysis Session 3 — Acquisition, Calibration and Peak Assignment Session 4 — Mini Capstone: Comparative MALDI vs LC–ESI Profiling

Session 1

Fee: Rs 8800

MALDI–TOF Fundamentals for Oligosaccharides

MALDI–TOF hardware and ion formation basics

laser, target plate and flight tube time of flight principles reflectron vs linear modes

Matrix selection for oligosaccharide analysis

DHB and CHCA options matrix solubility and purity additives and salt effects

Sample spotting and co crystallization strategies

dried droplet vs thin layer homogeneity and hot spot control basic troubleshooting tips

Session 2

Fee: Rs 11800

LC–ESI Workflows for Oligosaccharide Analysis

ESI source setup and tuning for glycans and oligosaccharides

nebulizer and gas settings capillary voltage and temperature minimizing in source fragmentation

LC configurations for LC–ESI oligosaccharide runs

isocratic vs gradient approaches HILIC and RP options injection solvent considerations

Direct infusion and LC–ESI comparison

sensitivity and throughput matrix and salt tolerance when to choose which workflow

Session 3

Fee: Rs 14800

Acquisition, Calibration and Peak Assignment

Acquisition parameters and method templates

mass range and resolution settings laser power and shot count in MALDI scan speed in LC–ESI MS

Calibration routines and quality checks

external and internal calibrants calibration frequency mass accuracy assessment

Peak picking, deisotoping and adduct recognition

isotope patterns for oligosaccharides Na and K adduct series linking m/z to compositions

Session 4

Fee: Rs 18800

Mini Capstone: Comparative MALDI vs LC–ESI Profiling

Designing a small comparative experiment

Theory plus Practical

Acquiring MALDI–TOF and LC–ESI data for the same sample

instrument specific run plans QC and replicate strategy data export and backup

Deliverables: comparison summary and method notes

PDF or DOC comparison report checklist of key parameters recommendations for routine use

PDF

MALDI–TOF & LC–ESI for Oligosaccharide Analysis Training | Matrix MS, ESI Workflows & Data Handling

MALDI–TOF and LC–ESI for Oligosaccharide Analysis — Hands-on