Mass Spectrometry Fundamentals — Ionization and MS/MS — Hands-on

Develop a clear, instrument-level understanding of how mass spectrometers work. This module walks through ion generation, transmission, mass analysis and detection, with a focus on ionization methods and MS/MS fragmentation used in proteomics and structural MS applications.

Mass Spectrometry Fundamentals — Ionization and MS/MS

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Session Index

Session 1 — MS Architecture & Core Principles Session 2 — Ionization Techniques & Ion Optics Session 3 — MS/MS Fragmentation & Acquisition Modes Session 4 — Calibration, QC & Mini Capstone

Session 1

Fee: Rs 8800

MS Architecture & Core Principles

Mass spectrometry building blocks and signal flow

source analyzer detector

m/z, resolution and mass accuracy concepts

isotopic patterns dynamic range signal to noise

Main analyzer families and use cases (overview)

quadrupole / ion trap TOF Orbitrap / FTICR

Session 2

Fee: Rs 11800

Ionization Techniques & Ion Optics

Soft ionization methods used in proteomics

ESI nanoESI MALDI (concepts)

Spray formation, charge states and adducts

solvent composition flow rate and voltage charge envelopes

Ion transfer region and basic ion optics

capillary and skimmer focusing lenses vacuum and pumping stages

Session 3

Fee: Rs 14800

MS/MS Fragmentation & Acquisition Modes

Principles of tandem MS and fragment ion series

precursor selection b / y ions charge directed fragmentation

Main fragmentation methods (conceptual view)

CID / HCD ETD / ECD (overview) collision energy tuning

Acquisition strategies and scan modes (overview)

full scan and MS/MS DDA concepts DIA idea and windowing

Session 4

Fee: Rs 18800

Calibration, QC & Mini Capstone

Mass calibration, tuning and basic QC checks

Theory + Practical (demo / guided)

Recognizing common MS issues from spectra

adducts and contamination source pollution signatures low sensitivity patterns

Mini capstone: method concept sheet for a proteomics run

instrument setup summary ion source settings (outline) acquisition strategy sketch

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Mass Spectrometry Fundamentals Training | Ionization, Analyzers & MS/MS for Proteomics

Mass Spectrometry Fundamentals — Ionization and MS/MS — Hands-on