Metabolomics Foundations & Experimental Design — Hands-on

Learn how to go from a biological question to a statistically-sound metabolomics study plan. This module builds strong foundations in metabolomics concepts, readouts, and experimental design — including platform choice, cohort definition, confounder control, QC strategy, and power/sample-size thinking for NMR/LC–MS/GC–MS studies.

Metabolomics Foundations & Experimental Design

Help Desk · WhatsApp

Session Index

Session 1 — Metabolomics Concepts, Matrices & Readouts Session 2 — Platforms, Workflows & Measurement Strategy Session 3 — Experimental Design, Bias & Power Session 4 — Mini Capstone: Study Protocol & Metadata

Session 1

Fee: Rs 8800

Metabolomics Concepts, Matrices & Readouts

What is metabolomics? Scope, metabolome layers & study types

global vs targeted steady-state vs flux metabolomics vs lipidomics

Biological matrices & pre-analytical constraints (overview)

plasma / serum / urine tissue / cells / media stability considerations

Biological questions & hypothesis framing in metabolomics

disease vs control time-course / intervention pharmacometabolomics

Session 2

Fee: Rs 11800

Platforms, Workflows & Measurement Strategy

NMR vs LC–MS vs GC–MS — pros, cons & typical use cases

1H NMR LC–MS (HILIC / RP) GC–MS (derivatization)

Untargeted vs targeted designs & panel selection (overview)

discovery profiling validation panels isotope-labeled standards

High-level workflow mapping from sample to data matrix

sample intake acquisition peak table generation

Session 3

Fee: Rs 14800

Experimental Design, Bias & Power

Cohort design: inclusion criteria, matching & blocking

case–control longitudinal cross-over designs

Sources of bias & confounding in metabolomics

diet / fasting medication / circadian batch / run-order

Power, sample size & replication strategy (concepts)

biological vs technical replicates effect size thinking pilot studies

Session 4

Fee: Rs 18800

Mini Capstone: Study Protocol & Metadata

Translate a biological question into a full study blueprint

Theory + Practical

Randomization, blocking & batch layout for LC–MS/GC–MS runs

run-order planning QC injections reference samples

Deliverables: protocol, sample sheet & metadata dictionary

study protocol (PDF/Word) sample & batch sheet (CSV) MIxS / MSI-style metadata

PDF

Metabolomics Foundations & Experimental Design Training | NMR / LC–MS / GC–MS Study Planning

Metabolomics Foundations & Experimental Design — Hands-on