Metabolomics Foundations — Design & QC — Hands-on

Establish a strong foundation in metabolomics by focusing on what matters most at the start of any small-molecule study: design, biospecimen handling, QC strategy and platform selection. This module prepares you to plan reproducible GC–MS, LC–MS and NMR metabolomics experiments that can scale into downstream statistics, pathway analysis and flux modeling.

Metabolomics Foundations — Design & QC

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Session Index

Session 1 — Metabolomics Concepts & Study Design Session 2 — Biospecimens, Extraction & QC Strategy Session 3 — Platforms: GC–MS, LC–MS & NMR Basics Session 4 — Mini Capstone: Study & QC Blueprint

Session 1

Fee: Rs 8800

Metabolomics Concepts & Study Design

Metabolome, metabolomics and study types

targeted vs untargeted discovery vs validation cross-sectional vs longitudinal

Experimental design for metabolomics

biological vs technical variation replication, randomization, blocking confounders and covariates

Sample size, power and effect sizes

pilot vs definitive studies variance estimation balanced designs

Session 2

Fee: Rs 11800

Biospecimens, Extraction & QC Strategy

Biospecimen types and pre-analytical factors

serum / plasma / urine tissues and cell pellets fasting, time-of-day, diet

Sample collection, storage and extraction

SOPs and labelling freeze–thaw control protein precipitation and solvent mixes

QC/QA strategy for metabolomics

pooled QCs and study QCs blanks and carryover checks system suitability and internal standards

Session 3

Fee: Rs 14800

Platforms: GC–MS, LC–MS & NMR Basics

Instrument options and trade-offs

GC–MS vs LC–MS vs NMR sensitivity and coverage throughput and cost

Chromatography and ionization basics

RP / HILIC modes derivatization for GC–MS ESI, APCI and polarity switching

Acquisition and basic QA readouts

full scan vs SIM/MRM MS/MS overview retention time and peak shapes

Session 4

Fee: Rs 18800

Mini Capstone: Study & QC Blueprint

Use-case driven study design

disease vs control or intervention

Randomization, batching and QC layout

injection order plans QC frequency and placement blank and standard runs

Metadata, data dictionary and reporting checklist

minimal metadata fields sample manifest MIRAGE and FAIR readiness

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Metabolomics Foundations — Design & QC Training | Study Design, Biospecimens, QC & Platforms

Metabolomics Foundations — Design & QC — Hands-on