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Metagenome-Assembled Genomes: Curation & QA/QC Training | CheckM/GUNC, MIMAG, GTDB-Tk

NTHRYS >> Services >> Academic Services >> Training Programs >> Bioinformatics Training >> Microbiome, Metagenomics & Environmental Bioinformatics >> Metagenome-Assembled Genomes: Curation & QA/QC Training | CheckM/GUNC, MIMAG, GTDB-Tk

Metagenome-Assembled Genomes: Curation & QA/QC — Hands-on

Transform raw genome bins into high-confidence Metagenome-Assembled Genomes (MAGs) . You'll diagnose contamination, perform targeted re-binning and re-assembly, evaluate against MIMAG tiers, assign taxonomy with GTDB-Tk, and build reproducible, submission-ready reports that stand up to peer review.

Metagenome-Assembled Genomes: Curation & QA/QC
Help Desk · WhatsApp
Session 1
Fee: Rs 8800
Diagnostics & Curation Workflow
  • Initial MAG screening & triage
    • size/GC/contig count coverage variance marker completeness
  • Interactive refinement planning
    • Anvi'o bin plots depth & tetranucleotide split/merge strategies
  • Curation playbook & SOPs
    • versioning evidence tracking reproducible records
Session 2
Fee: Rs 11800
Contamination Detection & Cleanup
  • Metrics & tools for QA/QC
    • CheckM completeness/contamination GUNC chimerism CheckV (viral bins)
  • Manual & automated cleanup
    • re-binning (MetaBAT2/MaxBin2/CONCOCT) DASTool integration contig removal/keep lists
  • Targeted re-assembly & scaffolding
    • read recruitment gap-filling strain deconvolution (overview)
Session 3
Fee: Rs 14800
Taxonomy, Quality Tiers & Validation
  • Taxonomic assignment & typing
    • GTDB-Tk ANI/AAI & Mash phylogenomics (marker sets)
  • MIMAG tiers & evidence
    • tRNA/rRNA presence completeness thresholds strain heterogeneity
  • Final validation checklist
    • QC tables & plots red flag review sign-off criteria
Session 4
Fee: Rs 18800
Reporting & Submission Preparation
  • Reproducible MAG report
    • tables, plots & QC badges provenance & parameters
  • Packaging & metadata
    • FASTA/GBK + annotations MIxS/MIMAG fields readme & licenses
  • Submission prep (overview)
    • public repo readiness accession fields checklist data/metadata sync


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