Microbial Glycobiology — LPS Capsules & Host Interactions — Hands-on

Explore how microbial surface glycans including lipopolysaccharide (LPS) , capsules and other cell surface glycoconjugates regulate colonization, virulence and immune recognition. This module combines microbial glycobiology concepts with practical LC-MS, lectin and bioinformatics workflows to derive LPS and capsule signatures that can be linked to host response, vaccine design and diagnostics.

Microbial Glycobiology — LPS Capsules & Host Interactions

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Session Index

Session 1 — Foundations of Microbial Glycobiology Session 2 — LPS and Capsules — Structure & Analytics Session 3 — Host–Pathogen Glycan Interactions & Immunity Session 4 — Mini Capstone: LPS/Capsule Glycan Signatures

Session 1

Fee: Rs 8800

Foundations of Microbial Glycobiology

Microbial cell surface architecture and glycocalyx overview

Gram negative and Gram positive envelopes peptidoglycan and teichoic acids context outer membrane and periplasm basics

Main classes of microbial surface glycans

LPS and lipooligosaccharide capsular polysaccharides exopolysaccharides and biofilms

Functional roles in colonization, protection and signaling

immune evasion and camouflage stress resistance and environmental fitness interaction with microbiome and host

Session 2

Fee: Rs 11800

LPS and Capsules — Structure & Analytics

LPS domains and capsule structural themes

lipid A, core and O antigen overview capsule serotypes and K antigens charge, hydrophobicity and heterogeneity

Extraction and sample preparation workflows

hot phenol and detergent based extractions enzyme and chemical treatments removal of proteins and nucleic acids

Analytical platforms for LPS and capsules

SDS PAGE and staining strategies LC MS and MS/MS profiling options lectin and antibody based assays

Session 3

Fee: Rs 14800

Host–Pathogen Glycan Interactions & Immunity

Pattern recognition receptors and LPS capsule sensing

TLR4 and lipid A recognition complement pathways and capsules lectin pathways and collectins

Immune evasion and virulence strategies

mimicry of host glycans serum resistance and phagocytosis escape biofilm formation and persistence

Implications for vaccines and diagnostics

conjugate vaccine antigen selection serotyping and typing schemes glycan based diagnostic signatures

Session 4

Fee: Rs 18800

Mini Capstone: LPS/Capsule Glycan Signatures

Build a small LPS and capsule profiling workflow

from raw data to serotype or pattern calls

Summarize microbial glycan signatures across isolates or conditions

heatmaps of LPS and capsule features clustering of strains or samples linking shifts to virulence or treatment

Deliverables and documentation set

curated LPS and capsule feature table figures ready for infection biology reports short narrative on host interaction insights

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Microbial Glycobiology — LPS Capsules & Host Interactions Training | LPS, Capsules & Host–Pathogen Glycans

Microbial Glycobiology — LPS Capsules & Host Interactions — Hands-on