Microbiome Foundations and Experimental Design — Hands-on

Gain a rigorous grounding in microbiome biology and contamination aware study design. You will plan sampling, storage, sequencing strategy and metadata so that downstream amplicon and shotgun analyses are robust, reproducible and meaningful for clinical, environmental and industrial applications.

Microbiome Foundations and Experimental Design

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Session Index

Session 1 — Microbiome Concepts & Host Environments Session 2 — Study Design, Sampling & Controls Session 3 — Sequencing Strategies, Metadata & Power Session 4 — Mini Capstone: Study Blueprint

Session 1

Fee: Rs 8800

Microbiome Concepts & Host Environments

Microbiome, metagenome and microbial ecosystems

gut / oral / skin / environmental commensal vs pathogen dysbiosis and resilience

Ecological concepts for microbiome data

richness and evenness alpha and beta diversity niche and keystone species

Application landscapes

clinical and public health food and agri systems environment and wastewater

Session 2

Fee: Rs 11800

Study Design, Sampling & Controls

Cohort design and hypotheses

cross sectional vs longitudinal case control and nested designs confounders and covariates

Sampling strategy and preservation

swabs / stool / soil / water cold chain and stabilizing buffers field logistics and lab intake

Controls and contamination sources

negative controls and blanks spike ins and standards kit and lab contamination

Session 3

Fee: Rs 14800

Sequencing Strategies, Metadata & Power

Amplicon vs shotgun choices

16S / ITS / 18S short read WGS long read options

Depth, replicates and power

coverage and rarefaction thinking power and sample size planning technical vs biological replicates

Metadata design and MIxS aware capture

questionnaires and CRFs ontologies and controlled vocabularies MIxS and reporting checklists

Session 4

Fee: Rs 18800

Mini Capstone: Study Blueprint

Drafting a complete microbiome study protocol

Theory plus practical

Risk registers and QC checklists

pre analytical analytical post analytical

Deliverables: protocol pack and metadata templates

protocol document flow diagrams metadata templates and codebook

PDF

Microbiome Foundations and Experimental Design Training | Study Design, Sampling, Contamination Control

Microbiome Foundations and Experimental Design — Hands-on