Microbiome Study Design & Sample Processing — Hands-on

Plan and execute end-to-end sampling for microbiome research across clinical, environmental, food, and aquaculture settings. You'll design studies, select sampling frames, implement contamination-aware collection and preservation, perform nucleic-acid extraction and QC, and prepare MIxS-ready metadata. Includes a mini pilot run to validate your workflow.

Microbiome Study Design & Sample Processing

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Session Index

Session 1 — Study Design & Sampling Strategy Session 2 — Collection, Preservation & Cold Chain Session 3 — DNA/RNA Extraction & QC Session 4 — MIxS Metadata & Pilot Run

Session 1

Fee: Rs 8800

Study Design & Sampling Strategy

Objectives, hypotheses & sampling frames

clinical / soil / water / air spatial–temporal design replicates & power

Bias & contamination controls

blanks / spikes randomization batching

Ethics & permits

consent & de-identification chain of custody

Session 2

Fee: Rs 11800

Collection, Preservation & Cold Chain

Sampling methods by matrix

stool / swabs soil / sediment water / air filters

Preservation & transport

RNAlater / OMNIgene dry ice / LN2 stabilizers & time-to-freeze

Field QA/QC & documentation

field blanks labels & barcodes SOPs

Session 3

Fee: Rs 14800

DNA/RNA Extraction & QC

Lysis & inhibitor removal

bead-beating kit selection low biomass care

Quality control metrics

A260/280 & A260/230 Qubit / TapeStation process controls

Amplicon vs shotgun readiness

amplicon targets fragment size host DNA depletion

Session 4

Fee: Rs 18800

MIxS Metadata & Pilot Run

MIxS checklists & sample sheets

FAIR & submission-ready

Randomization & barcoding plan

plate maps index strategy batch tracking

Pilot run & quick health-check

QIIME 2 / FastQC read stats & controls go/no-go criteria

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Microbiome Study Design & Sample Processing Training | Sampling, Preservation, Extraction, MIxS

Microbiome Study Design & Sample Processing — Hands-on