Multiple Testing, FDR Control & Batch Effects — Hands-on

Learn how to control false positives in high dimensional omics while handling real world batch effects. This module covers family wise error, FDR procedures, independent filtering and practical batch correction, implemented in R and Python using real gene expression and other omics datasets.

Multiple Testing, FDR Control & Batch Effects

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Session Index

Session 1 — Multiple Testing & Family-Wise Error Session 2 — FDR Methods in High-Dimensional Omics Session 3 — Batch Effects: Detection & Correction Session 4 — Integrated Workflow: DE, FDR & Batches

Session 1

Fee: Rs 8800

Multiple Testing & Family-Wise Error

Why multiple testing matters in omics

thousands of features false positives vs discovery FWER vs FDR

Classical FWER control

Bonferroni & Holm family definition conservative vs powerful

Permutation & resampling ideas

maxT/minP approaches null distributions computational considerations

Session 2

Fee: Rs 11800

FDR Methods in High-Dimensional Omics

Benjamini–Hochberg and variants

BH & BY procedures q-values & local FDR dependent tests & structure

Independent filtering & weighting

filtering low-count features p-value weighting trade-off: power vs bias

Practical omics workflows

gene lists with FDR control volcano & MA plots reporting thresholds & caveats

Session 3

Fee: Rs 14800

Batch Effects: Detection & Correction

Sources and signatures of batch effects

lab / run / site effects PCA and clustering diagnostics metadata audits

Correction strategies

ComBat style adjustments including batch in models sva / RUV style methods

Common pitfalls & sensitivity checks

over-correction risks confounded design issues pre/post batch QC panels

Session 4

Fee: Rs 18800

Integrated Workflow: DE, FDR & Batches

End-to-end case study with real data

from raw matrix to gene list

Reproducible R & Python pipelines

R limma / edgeR style GLMs Python pandas & statsmodels scripts & notebooks

Deliverables: QC, FDR and batch report

pre/post batch plots FDR controlled hit list workflow documentation

PDF

Multiple Testing, FDR Control & Batch Effects Training | Biostatistics & Omics Analytics

Multiple Testing, FDR Control & Batch Effects — Hands-on