NMR Metabolomics 1D & 2D Processing and Quantification — Hands-on

Get hands on with 1D and 2D NMR based metabolomics. You will configure acquisition parameters, perform water suppression, phase and baseline correction, binning and alignment, and move towards spectral assignment and quantitative workflows suitable for biofluids, tissues and cell extracts.

NMR Metabolomics 1D & 2D Processing & Quantification

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Session Index

Session 1 — NMR Metabolomics Principles & 1D Acquisition Session 2 — 1D Processing, Baseline, Phase & Binning Session 3 — 2D Experiments & Spectral Assignment Session 4 — Quantification, QC & Data Export

Session 1

Fee: Rs 8800

NMR Metabolomics Principles & 1D Acquisition

NMR basics for metabolomics users

chemical shift and relaxation pulse sequences overview 1D versus 2D information

Sample preparation and referencing for NMR

biofluids, extracts and buffers reference compounds and lock shimming and temperature control

1D acquisition parameters and water suppression

number of scans and relaxation delays pulse angle and spectral width water suppression schemes for biofluids

Session 2

Fee: Rs 11800

1D Processing, Baseline, Phase & Binning

Raw FID handling and Fourier transform

apodization and zero filling frequency domain basics common artefacts

Phase and baseline correction strategies

manual versus automatic phase baseline distortions and fitting quality checks before quantification

Alignment, binning and spectral regions for analysis

chemical shift alignment concepts fixed versus adaptive binning excluding water and artefact regions

Session 3

Fee: Rs 14800

2D Experiments & Spectral Assignment

Key 2D NMR experiments for metabolomics

COSY and TOCSY concepts HSQC and HMBC overview when to use which experiment

Processing and visualising 2D spectra

apodization and zero filling in 2D contour plots and projections peak picking strategies

Spectral assignment using libraries and 2D correlations

matching to reference spectra using 2D correlations for confirmation building assignment tables for metabolites

Session 4

Fee: Rs 18800

Quantification, QC & Data Export

Quantification strategies in NMR metabolomics

integral based approaches internal and external standards targeted versus global quantification

QC metrics and reproducibility checks for NMR data

line shape and SNR criteria reference peak stability technical replicates and drift

Exporting NMR metabolomics data for downstream analysis

binned matrices and peak tables linking spectra to metadata ready to use inputs for statistics pipelines

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NMR Metabolomics 1D & 2D Processing and Quantification Training | Acquisition, Processing & Quant Workflows

NMR Metabolomics 1D & 2D Processing and Quantification — Hands-on