Non-coding RNA Analysis (miRNA, lncRNA) — Hands-on

Gain expertise in non-coding RNA analysis—small RNA QC and discovery, miRNA quantification and targets, lncRNA discovery and coding potential, and reproducible ncRNA pipelines.

Non-coding RNA Analysis (miRNA, lncRNA)

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Session Index

Session 1 — Small RNA QC & miRNA Discovery Session 2 — miRNA Quantification & Targets Session 3 — lncRNA Discovery & Annotation Session 4 — Mini Capstone: ncRNA Network

Session 1

Fee: Rs 6300

Small RNA QC & miRNA Discovery

Library QC, adapters, size-selection and metadata setup

Theory

Small RNA read processing & alignment best practices

FastQC Cutadapt Bowtie STAR

Known/novel miRNA discovery, isomiRs & QC metrics

miRDeep2 miRge3.0 isomiRs

Session 2

Fee: Rs 8400

miRNA Quantification & Targets

Count matrices, normalization & differential expression

DESeq2 edgeR limma

Target prediction, validation databases & enrichment views

TargetScan miRanda miRTarBase

Integration with mRNA expression & pathway overlays

clusterProfiler KEGG/Reactome

Session 3

Fee: Rs 11200

lncRNA Discovery & Annotation

Transcript assembly, merging & reference comparison

StringTie gffcompare FEELnc

Coding potential & classification (intergenic, antisense)

CPC2 CPAT CNCI

Interaction prediction & functional context

lncTar RIsearch2 lncLocator

Session 4

Fee: Rs 14000

Mini Capstone: ncRNA Network

Design an end-to-end ncRNA pipeline with provenance

Theory

Integrate miRNA–mRNA–lncRNA interactions & visualize networks

Cytoscape igraph Enrichr

Automate QC→discovery→targets→reports with dashboards

Snakemake Conda/Mamba Quarto/MultiQC

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Non-coding RNA (miRNA, lncRNA) Analysis Training | Discovery, Targets, Coding Potential & Networks

Non-coding RNA Analysis (miRNA, lncRNA) — Hands-on