Oxylipins, Eicosanoids & Signaling Lipids — Hands-on

Learn how to design and analyse oxylipin and eicosanoid–focused lipidomics studies. This module covers biochemical pathways, targeted LC–MS/MS acquisition, MRM panel design, quantification strategies and biological interpretation so that you can deliver decision ready signaling lipid readouts for inflammation, cardiovascular, metabolic and immunology projects.

Oxylipins, Eicosanoids & Signaling Lipids

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Session Index

Session 1 — Pathways, Classes & Biology of Oxylipins Session 2 — Sample Prep & LC–MS/MS Method Setup Session 3 — Targeted Panels, Quantitation & QC Session 4 — Mini Capstone: Disease Focused Oxylipin Panel

Session 1

Fee: Rs 8800

Pathways, Classes & Biology of Oxylipins

Oxylipin and eicosanoid biochemistry overview

PUFA substrates (AA, EPA, DHA) COX / LOX / CYP pathways pro and anti inflammatory mediators

Classes, nomenclature and structure families

prostaglandins, leukotrienes, HETEs resolvins, protectins, maresins naming, isomers and stereochemistry

Biological and clinical application areas

inflammation and immune regulation cardio metabolic disease and obesity neuroinflammation and pain

Session 2

Fee: Rs 11800

Sample Prep & LC–MS/MS Method Setup

Pre analytical considerations for signaling lipids

anticoagulants and matrix selection handling to avoid ex vivo formation storage, stability and freeze thaw

SPE and extraction strategies

solid phase extraction cartridges recovery checks and matrix effects internal standard spiking schemes

LC–MS/MS conditions for oxylipin panels

RP LC gradients and column choice negative mode ESI specifics source tuning for labile species

Session 3

Fee: Rs 14800

Targeted Panels, Quantitation & QC

MRM / SRM panel design for oxylipins

precursor product ion pairs collision energy optimisation scheduling and dwell times

Calibration and quantitative validation

isotope labelled internal standards linearity, LOD/LOQ and accuracy intra and inter day precision

QC metrics and longitudinal drift monitoring

pooled QC injections and trend plots acceptance criteria for runs and analytes documentation for regulated studies

Session 4

Fee: Rs 18800

Mini Capstone: Disease Focused Oxylipin Panel

Designing an oxylipin panel for a use case

cardiometabolic, inflammatory or neuro disease

End to end analysis on a teaching dataset

from raw files to quantified oxylipins summary plots and ratio metrics linking changes to pathways and outcomes

Deliverables: panel table, QC summary & methods text

list of analytes with transitions acceptance and performance metrics ready to edit methods and results section

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Oxylipins, Eicosanoids & Signaling Lipids Training | Targeted LC–MS/MS Panels & Biology

Oxylipins, Eicosanoids & Signaling Lipids — Hands-on