Protein Network Analysis and Functional Annotation — Hands-on

Learn the conceptual workflow of protein network analysis and functional annotation. This module focuses on how to move from protein lists to interaction networks, interpret hubs and modules, and use Gene Ontology (GO) and pathway enrichment concepts to generate biological stories from proteomics or gene level datasets.

Protein Network Analysis and Functional Annotation

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Session Index

Session 1 — Protein Interaction & Network Biology Foundations Session 2 — Building & Exploring Protein Networks Session 3 — Functional Annotation & Enrichment Concepts Session 4 — Mini Capstone: From Protein List to Network Narrative

Session 1

Fee: Rs 8800

Protein Interaction & Network Biology Foundations

Protein–protein interactions and network view of biology

binary vs complex interactions physical vs functional associations interactome idea

Basic graph concepts for protein networks (idea level)

nodes and edges thinking degree and hubs modules and communities (overview)

Sources of interaction and functional association data (concepts)

curated PPI databases idea text mining and co expression concepts confidence scores thinking

Session 2

Fee: Rs 11800

Building & Exploring Protein Networks (Concepts)

From protein list to interaction network at high level

mapping identifiers to network resources filtering by confidence concepts sub networks around proteins of interest

Exploring topology and key nodes conceptually

hubs and bottlenecks ideas shortest path and connectivity concepts cluster and module overview

Visual thinking for network layouts and annotations

node color and size for attributes edge width and type concepts practical readability considerations

Session 3

Fee: Rs 14800

Functional Annotation & Enrichment Concepts

Gene Ontology (GO) and annotation concepts

biological process ideas molecular function and cellular component annotation evidence codes overview

Pathway and functional enrichment analysis at idea level

over representation thinking multiple testing and FDR awareness Reactome and KEGG style pathway concepts

Overlaying enrichment and annotations onto networks

coloring modules by enriched terms highlighting key pathways and hubs connecting patterns to hypotheses

Session 4

Fee: Rs 18800

Mini Capstone: From Protein List to Network Narrative

Conceptual workflow from differential protein list to network story

Theory + Practical (planning oriented)

Designing simple enrichment and network interpretation steps

selecting network and annotation resources deciding cutoffs and filters (concepts) sketching expected figures and tables

Mini capstone deliverables (concept and report focused)

network and enrichment summary outline short biological narrative template list of follow up experiment ideas

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Protein Network Analysis & Functional Annotation Training | PPI Networks, GO & Pathway Concepts

Protein Network Analysis and Functional Annotation — Hands-on