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Proteomics Foundations & Experimental Design Training | LC-MS/MS Study Design & QC

NTHRYS >> Services >> Academic Services >> Training Programs >> Bioinformatics Training >> Proteomics, Mass Spectrometry & PTM Analytics >> Proteomics Foundations & Experimental Design Training | LC-MS/MS Study Design & QC

Proteomics Foundations & Experimental Design — Hands-on

Build a strong foundation in modern LC–MS/MS based proteomics and learn how to design robust experiments from biological question to acquisition strategy and quality control. This module focuses on experimental planning, sample handling and design choices so that you can confidently select platforms, controls and replicates for discovery, targeted and clinical proteomics studies.

Proteomics Foundations & Experimental Design
Help Desk · WhatsApp
Session 1
Fee: Rs 8800
Proteomics Concepts & Workflows
  • Proteome organization and measurable proteomics readouts
    • bottom-up vs top-down shotgun vs targeted discovery vs verification
  • Core LC–MS/MS workflow and terminology
    • ionization and analyzers MS1 / MS2 peptide-centric view
  • Study questions that are suitable for proteomics
    • biomarker discovery mechanistic signalling time course and perturbation
Session 2
Fee: Rs 11800
Samples, Prep & Experimental Factors
  • Sample types and proteome complexity
    • cells and tissues biofluids subcellular fractions
  • Protein extraction, digestion and cleanup choices
    • detergents and chaotropes in-solution vs in-gel desalting and enrichment
  • Pre-analytical variables and batch effects
    • collection and storage freeze–thaw cycles randomization strategies
Session 3
Fee: Rs 14800
LC-MS/MS Modes & Quant Strategies
  • Acquisition modes and when to use them
    • DDA conceptually DIA conceptually targeted SRM/PRM overview
  • Quantitative designs and dynamic range
    • label free intensity isobaric tags concept internal standards
  • Instrument time budgeting and throughput
    • gradients and cycle time sample multiplexing pilot runs
Session 4
Fee: Rs 18800
Experimental Design & Case Studies
  • Replicates, blocking and controls in proteomics
    • theory plus planning exercise
  • QC strategy and monitoring over a run
    • system suitability pooled QC spikes and standards
  • Designing a small proteomics study plan
    • study worksheet sample map instrument queue outline


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