Proteomics Fundamentals & Sample Preparation — Hands-on

Build a strong foundation in modern proteomics by learning how to design experiments and prepare high quality protein and peptide samples for LC–MS/MS. You will practice extraction, solubilization, reduction and alkylation, digestion, desalting and QC workflows that directly impact identification and quantitation.

Proteomics Fundamentals & Sample Preparation

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Session Index

Session 1 — Proteomics Concepts & Experimental Design Session 2 — Protein Extraction, Lysis & Cleanup Session 3 — Digestion, Peptide Cleanup & Quant Session 4 — QC, Troubleshooting & Mini Capstone

Session 1

Fee: Rs 8800

Proteomics Concepts & Experimental Design

Introduction to proteomics and LC–MS based workflows

bottom up vs top down discovery vs targeted label free vs labeled

Study design and sample collection strategy

biological vs technical replicates batch effects randomization & blocking

Overview of proteomics toolchain

sample prep lab workflow LC–MS/MS overview data analysis outline

Session 2

Fee: Rs 11800

Protein Extraction, Lysis & Cleanup

Sample types and lysis strategies

cells and tissues body fluids detergent and chaotrope based lysis

Protein solubilization and interference removal

urea and guanidine removal of SDS, salts, lipids precipitation methods

Protein quantitation and integrity checks

BCA / Bradford / UV SDS PAGE QC aliquoting and storage

Session 3

Fee: Rs 14800

Digestion, Peptide Cleanup & Quant

Reduction, alkylation and enzymatic digestion

DTT and IAA workflow trypsin and alternative enzymes in solution and in gel digestion

Peptide desalting and cleanup for LC–MS

C18 stage tips and SPE drying and reconstitution carryover control

Peptide level QC and normalization planning

peptide yield checks spike in standards concepts loading strategies

Session 4

Fee: Rs 18800

QC, Troubleshooting & Mini Capstone

Common sample prep pitfalls and troubleshooting

Theory + Practical

Preparing samples for a real LC–MS run

checklist and documentation simple run order plan handover to core facility

Mini capstone: design to ready to inject samples

worksheet and protocol template QC summary future analysis plan

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Proteomics Fundamentals & Sample Preparation Training | Protein Extraction, Digestion & LC–MS Sample Prep

Proteomics Fundamentals & Sample Preparation — Hands-on