PTM Analysis — Phospho Acetyl Ubiquityl Glyco — Hands-on

Learn how to design, execute and interpret post translational modification centric proteomics studies with a focus on phosphorylation, acetylation, ubiquitylation and glycosylation. From enrichment strategies and LC MS acquisition settings to PTM site localization, FDR control, motif analysis and pathway level interpretation, you will build complete PTM analysis workflows that are ready for publication.

PTM Analysis — Phospho Acetyl Ubiquityl Glyco

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Session Index

Session 1 — PTM Biology, Types & Study Design Session 2 — Phosphoproteomics Workflows & Enrichment Session 3 — Acetyl, Ubiquityl & Glyco Workflows Session 4 — Site Localization, Motifs & Reporting

Session 1

Fee: Rs 12320

PTM Biology, Types & Study Design

PTM landscape and functional roles

phospho, acetyl, ubiquitin, glyco signal transduction stability and localization

PTM centric experimental design

stimulus and time course inhibitors and controls replicates and depth planning

Sample prep considerations for PTMs

phosphatase and deacetylase inhibitors denaturation and digestion conditions avoiding PTM loss and artefacts

Session 2

Fee: Rs 16520

Phosphoproteomics Workflows & Enrichment

Phosphopeptide enrichment strategies

IMAC and TiO2 concepts mono vs multi step enrichment pH and buffer optimization

LC MS settings for phosphoproteomics

collision energy tuning neutral loss behavior scan ranges and dynamic exclusion

Phosphosite centric data handling

phospho tables vs protein tables class I site concepts handling multi phospho peptides

Session 3

Fee: Rs 20720

Acetyl, Ubiquityl & Glyco Workflows

Acetyl and ubiquitin remnant enrichment

antibody based enrichment K epsilon GG remnant peptides on bead digestion ideas

Glycopeptide analysis basics

lectin and HILIC enrichment N linked vs O linked focus glycan vs peptide fragmentation balance

Search and annotation for non phospho PTMs

variable mods and search space control open search style PTM discovery ideas PTM annotation tools overview

Session 4

Fee: Rs 26320

Site Localization, Motifs & Reporting

PTM site localization and scoring

localization scores and probabilities class I site definitions site level FDR concepts

Motif and pathway level analysis

sequence logo and motif finders kinase substrate enrichment ideas mapping to KEGG and Reactome

PTM reports, repositories and checklists

PTM specific result tables figure panels for reviewers PRIDE and ProteomeXchange PTM submissions

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PTM Analysis — Phospho Acetyl Ubiquityl Glyco Training | Enrichment, Site Localization, Motifs & Pathways

PTM Analysis — Phospho Acetyl Ubiquityl Glyco — Hands-on