PTM Proteomics — Phospho Glyco Ubiquityl Scan — Hands-on

Learn how to plan and interpret post translational modification proteomics with emphasis on phosphorylation, glycosylation and ubiquitylation. This module covers conceptual enrichment strategies, PTM aware database searches, localization confidence, site level quantification and how to turn PTM maps into hypotheses about signaling, regulation and pathway rewiring.

PTM Proteomics — Phospho Glyco Ubiquityl Scan

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Session Index

Session 1 — PTM Proteomics Foundations & Strategy Session 2 — Phospho Enrichment & MS/MS Concepts Session 3 — Glyco & Ubiquityl PTMs & Data Interpretation Session 4 — Study Design, QC & Biological Readouts

Session 1

Fee: Rs 8800

PTM Proteomics Foundations & Strategy

Overview of major PTMs in proteomics studies

phosphorylation overview glycosylation overview ubiquitylation overview

PTM vs global proteome experiment logic

motivation for PTM focused workflows enrichment needs and tradeoffs sample amount and coverage considerations

Conceptual PTM study workflows end to end

from lysis to enriched peptides PTM aware search and localization site level quantification and interpretation

Session 2

Fee: Rs 11800

Phospho Enrichment & MS/MS Concepts

Phosphopeptide enrichment strategies (conceptual)

IMAC and metal oxide affinity ideas combining global and phospho fractions sources of enrichment bias

MS/MS behaviour of phosphopeptides

neutral loss concepts fragment ion patterns around sites choice of fragmentation modes idea

PTM aware search space and localization thinking

variable modification concept controlling combinatorial explosion site localization score intuition

Session 3

Fee: Rs 14800

Glyco & Ubiquityl PTMs & Data Interpretation

Glyco PTM concepts for proteomics scans

N linked and O linked overview released glycan vs glycopeptide views fragmentation pattern intuition

Ubiquityl and digly PTM concepts

K GG remnant idea enrichment logic for ubiquityl peptides chain type and site mapping concepts

From site lists to biology and pathways (conceptual)

site centric vs protein centric views mapping to pathways and networks patterns of regulation across conditions

Session 4

Fee: Rs 18800

Study Design, QC & Biological Readouts

Designing PTM focused studies on paper

theory plus planning worksheet

QC for PTM enrichment and data quality

enrichment efficiency indicators site localization confidence patterns replicate consistency concepts

Summarizing and reporting PTM results

tables of sites and motifs pathway and network style figures methods descriptions for manuscripts

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PTM Proteomics — Phospho Glyco Ubiquityl Scan Training | Site Level PTM Mapping

PTM Proteomics — Phospho Glyco Ubiquityl Scan — Hands-on