Sample Prep & QC for Metabolomics & Lipidomics — Hands-on

Get sample preparation and quality control right before you ever run the instrument. This module focuses on pre-analytical variables, extraction workflows, internal standards, and end-to-end QC design for metabolomics and lipidomics so that downstream peak picking, statistics, and biology sit on a robust analytical foundation.

Sample Prep & QC for Metabolomics & Lipidomics

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Session Index

Session 1 — Pre-Analytical Variables & Sample Collection Session 2 — Extraction Workflows & Internal Standards Session 3 — QC Strategy, Batches & System Suitability Session 4 — Mini Capstone: SOP & QC Map for a Study

Session 1

Fee: Rs 8800

Pre-Analytical Variables & Sample Collection

Pre-analytical phase and its impact on metabolite stability

temperature & freeze–thaw time to processing additives & tubes

Collection protocols for key matrices

plasma vs serum urine & fecal samples tissues, cells & media

Aliquoting, storage and chain-of-custody basics

aliquot strategy biobank labelling metadata capture

Session 2

Fee: Rs 11800

Extraction Workflows & Internal Standards

Protein precipitation and metabolite extraction for LC–MS/GC–MS

methanol / acetonitrile biphasic extraction phase separation tips

Lipid extraction strategies and carryover prevention (overview)

Folch / Bligh & Dyer SPE clean-up plastic vs glassware

Internal standards, surrogates and spike-in strategy

isotopically labelled standards recovery assessment normalization anchors

Session 3

Fee: Rs 14800

QC Strategy, Batches & System Suitability

QC sample types and their roles in metabolomics runs

pooled QC process blanks solvent blanks & spikes

Batch layout, run-order design and drift monitoring

interleaved QCs conditioning injections carryover checks

System suitability and basic acceptance criteria (concepts)

retention time windows peak shape & intensity CVs in QC samples

Session 4

Fee: Rs 18800

Mini Capstone: SOP & QC Map for a Study

Drafting a sample prep SOP for a chosen matrix and platform

Theory + Practical

Designing QC and batch layout for a real or mock cohort

placement of QCs blank and spike strategy accept / repeat rules

Deliverables: SOP, batch sheet and QC checklist

sample prep SOP (PDF/Word) run-order & batch sheet (CSV) QC and suitability checklist

PDF

Sample Prep & QC for Metabolomics & Lipidomics Training | Extraction Workflows & QC Design

Sample Prep & QC for Metabolomics & Lipidomics — Hands-on