Sample Prep for Metabolomics & Lipidomics — Hands-on

Learn how to go from biological material to extract-ready vials in a way that preserves small molecules and enables reproducible metabolomics and lipidomics data. This module focuses on biospecimen handling, matrix-specific extraction protocols, lipid-focused workflows and QC-aware sample preparation that integrates cleanly with GC–MS, LC–MS and NMR pipelines.

Sample Prep for Metabolomics & Lipidomics

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Session Index

Session 1 — Biospecimen Types & Collection Workflows Session 2 — Metabolite Extraction Protocols & Optimization Session 3 — Lipid Extraction & Class-Specific Enrichment Session 4 — Mini Capstone: End-to-End Sample Prep Plan

Session 1

Fee: Rs 8800

Biospecimen Types & Collection Workflows

Overview of matrices for metabolomics and lipidomics

serum / plasma / whole blood urine, saliva, CSF tissues and cultured cells

Pre-analytical variables and collection SOPs

anticoagulants and collection tubes clotting, centrifugation and processing time temperature control and transport

Aliquoting, labelling and chain-of-custody

barcodes and manifests biobanking practices freeze–thaw cycle control

Session 2

Fee: Rs 11800

Metabolite Extraction Protocols & Optimization

Protein precipitation and solvent systems

methanol / acetonitrile protocols cold extraction and quenching handling high-protein matrices

Matrix-dependent extraction workflows

biofluids vs tissues vs cells homogenization and bead beating solid phase extraction basics

Internal standards and recovery checks

stable isotope labelled standards spike-in strategies recovery and matrix effect evaluation

Session 3

Fee: Rs 14800

Lipid Extraction & Class-Specific Enrichment

Classical and modern lipid extraction methods

Folch and Bligh–Dyer MTBE based extraction phase separation and fractionation

Lipid class enrichment and clean up

phospholipids, sphingolipids, neutral lipids solid phase and silica cartridges minimizing oxidation and degradation

Storage, evaporation and reconstitution for LC–MS

nitrogen / vacuum evaporation solvent choices for injection lipid QC samples and checks

Session 4

Fee: Rs 18800

Mini Capstone: End-to-End Sample Prep Plan

Design a sample prep workflow for a use case

clinical, nutrition or cell culture study

QC- and contamination-aware layout

blanks, QCs and standards carryover and background control robustness and reproducibility checks

Final sample prep SOP and checklist deliverable

stepwise SOP document reagent and consumables list safety and waste handling notes

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Sample Prep for Metabolomics & Lipidomics Training | Biospecimens, Extraction & QC-Aware Workflows

Sample Prep for Metabolomics & Lipidomics — Hands-on