Sample Prep — Protein Extraction, Digestion and Cleanup — Hands-on

Go from complex biological samples to LC–MS/MS ready peptide mixtures with confidence. This module focuses on the theory and practical decision-making behind protein extraction, solubilization, digestion and cleanup so that your downstream identification and quantification are not compromised by poor sample quality.

Sample Prep — Protein Extraction, Digestion and Cleanup

Help Desk · WhatsApp

Session Index

Session 1 — Lysis & Protein Extraction Basics Session 2 — Reduction, Alkylation & Digestion Session 3 — Cleanup, Fractionation & Enrichment Session 4 — QC, Troubleshooting & Study Templates

Session 1

Fee: Rs 8800

Lysis & Protein Extraction Basics

Overview of sample types and matrix challenges

cells and tissues biofluids membrane rich samples

Lysis and solubilization strategies (conceptual)

mechanical vs chemical lysis detergents and chaotropes protease and phosphatase inhibitors

Protein recovery, clarification and storage considerations

clarification and removal of debris buffer compatibility with LC–MS/MS aliquoting and freeze protection

Session 2

Fee: Rs 11800

Reduction, Alkylation & Digestion Chemistry

Disulfide bond management and denaturation choices

why reduction and alkylation impact on peptide mapping common pitfalls

Enzymatic digestion strategies (theory level)

trypsin and other proteases on pellet vs in solution logic missed cleavages and sequence coverage

Designing digestion workflows for different sample types

low vs high complexity samples time and temperature constraints compatibility with labeling strategies

Session 3

Fee: Rs 14800

Cleanup, Fractionation & Enrichment

Why cleanup is essential before LC–MS/MS

removing salts and detergents improving sensitivity and robustness carryover considerations

Concepts of solid phase cleanup and fractionation

reversed phase at peptide level offline fractionation logic orthogonality of separations

Enrichment strategies for targeted sub proteomes (overview)

depletion of high abundance species basic PTM and peptide enrichment concepts trade offs between depth and throughput

Session 4

Fee: Rs 18800

QC, Troubleshooting & Study Templates

Assessing protein and peptide quality prior to LC–MS/MS

theory plus checklist exercise

Common failure modes and how to reason about them

low protein yield poor digestion patterns carryover and contamination indicators

Building sample prep sections for a proteomics study plan

template for methods sections sample mapping sheets handoff checklists to MS core

PDF

Sample Prep — Protein Extraction, Digestion and Cleanup Training | LC-MS/MS Ready Proteomics Samples

Sample Prep — Protein Extraction, Digestion and Cleanup — Hands-on