Sample Preparation, QC & Matrix Effects — Hands-on

Develop a rigorous approach to sample preparation, QC and matrix effect management for LC MS based metabolomics and lipidomics. You will connect biospecimen handling, extraction protocols, QC samples and system suitability to downstream data quality and biological interpretation.

Sample Preparation, QC & Matrix Effects

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Session Index

Session 1 — Biospecimen Handling & Pre Analytical Variables Session 2 — Extraction Protocols & Sample Cleanup Session 3 — QC Design & System Suitability Session 4 — Matrix Effects, Stability & Robustness

Session 1

Fee: Rs 8800

Biospecimen Handling & Pre Analytical Variables

Biospecimen types and collection strategies

plasma and serum and urine cells and tissues and biofluids field versus clinic workflows

Pre analytical variables and control

fasting and time of day anticoagulants and additives processing delays and storage

Aliquoting and freeze thaw management

aliquot strategy freeze thaw cycles cold chain logistics

Session 2

Fee: Rs 11800

Extraction Protocols & Sample Cleanup

Protein precipitation and solvent systems

methanol and acetonitrile mixes cold precipitation sample to solvent ratios

Lipid extraction and phase partitioning

Bligh Dyer and Folch style single phase and biphasic safety and reproducibility

Cleanup and concentration steps

solid phase extraction basics dry down and reconstitution carryover reduction practices

Session 3

Fee: Rs 14800

QC Design & System Suitability

QC sample types and roles

pooled QC system suitability QC blank and solvent QC

Run order and batch layout

randomization within batches insertion of QC series conditioning and wash cycles

Monitoring drift and instrument health

intensity and RT trends precision metrics acceptance criteria plans

Session 4

Fee: Rs 18800

Matrix Effects, Stability & Robustness

Concepts and diagnosis of matrix effects

suppression and enhancement basics

Stability studies and stress conditions

bench top stability autosampler stability freeze thaw stability

Method robustness and documentation

critical step identification checklists and SOPs handover to data team

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Sample Preparation, QC & Matrix Effects Training | Biospecimen Handling, Extraction & Matrix Control

Sample Preparation, QC & Matrix Effects — Hands-on